Protein Info for Dsui_0805 in Dechlorosoma suillum PS

Annotation: primosomal protein N''

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 674 PF17764: PriA_3primeBD" amino acids 5 to 103 (99 residues), 77.1 bits, see alignment E=2.3e-25 PF04851: ResIII" amino acids 131 to 278 (148 residues), 40.9 bits, see alignment E=6.4e-14 PF00270: DEAD" amino acids 133 to 300 (168 residues), 60.4 bits, see alignment E=5.6e-20 TIGR00595: primosomal protein N'" amino acids 158 to 672 (515 residues), 545.8 bits, see alignment E=4.6e-168 PF18319: PriA_CRR" amino acids 388 to 412 (25 residues), 35.3 bits, see alignment (E = 2.7e-12) PF00271: Helicase_C" amino acids 430 to 531 (102 residues), 27.7 bits, see alignment E=8.2e-10 PF18074: PriA_C" amino acids 575 to 671 (97 residues), 52.7 bits, see alignment E=1.9e-17

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 68% identity to dar:Daro_0033)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QHR1 at UniProt or InterPro

Protein Sequence (674 amino acids)

>Dsui_0805 primosomal protein N'' (Dechlorosoma suillum PS)
MPVIRVALDVPLHRQFDYLAPDAQPDDVGRRVRAPFGRGTRIGVITAVLGADEEGDLPLD
KLKAAEILRDDVPPLPPDWFRLTEFCSSYYQAPIGEVMLAALPVGLRRTDPPKARAVKAK
VKVEAPQVAPELTPGQAEAVAAVLAAGVGTAPRFAPFLLYGITGSGKTEVYLRLVEAALA
GGHQALLLVPEINLTPQLEERVRRRFPGVGLVSLHSELTEAARTRHWKAAFKGEARIVLG
TRLSIFTPLPDLGLIVVDEEHDPSFKQQDGMRYSARDVAVFRAWQRGVPIVLGSATPSLE
TWANATGEGLPRRYTLLTLADRANAAASLPPVRLLDTRREKLQDGLSAALLAALQVRLER
GEQSLVFLNRRGYAPVLACPSCGWISRCRRCAANLVLHLADRRLRCHHCGFENGIPRACP
SCGNQDIHPFGRGTQRLETTLQERFPEARILRVDRDSAKSRKQWEALAEKIHAGEADILV
GTQMLAKGHDFPRLTLVGVTGADAALFAADWRAPERLFAQLMQVGGRAGRAELPGEVLIQ
TEHPEHPLYGALQQHDFAAFAASQLEERRSAGFPPYSYQVMLRAEAPEMGPALEYLAQAR
ALGADLNGNGPVLLYDPVPMRMARRANLERAQLLAESTSRPALQAFLAEWRPLLEGIKAP
SRLRWHLEVDPLEF