Protein Info for Dsui_0686 in Dechlorosoma suillum PS

Annotation: tRNA nucleotidyltransferase/poly(A) polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 406 PF01743: PolyA_pol" amino acids 3 to 122 (120 residues), 61 bits, see alignment E=2.4e-20 PF12627: PolyA_pol_RNAbd" amino acids 147 to 209 (63 residues), 45.2 bits, see alignment E=1e-15 PF01966: HD" amino acids 226 to 308 (83 residues), 33.9 bits, see alignment E=5.4e-12

Best Hits

Swiss-Prot: 71% identical to CCA_DECAR: Multifunctional CCA protein (cca) from Dechloromonas aromatica (strain RCB)

KEGG orthology group: K00974, tRNA nucleotidyltransferase (CCA-adding enzyme) [EC: 2.7.7.72 3.1.3.- 3.1.4.-] (inferred from 71% identity to dar:Daro_4135)

MetaCyc: 54% identical to fused CCA tRNA nucleotidyltransferase/phosphohydrolase (Escherichia coli K-12 substr. MG1655)
TRNA-CYTIDYLYLTRANSFERASE-RXN [EC: 2.7.7.72]

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-, 3.1.4.-

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72 or 3.1.3.- or 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QGL6 at UniProt or InterPro

Protein Sequence (406 amino acids)

>Dsui_0686 tRNA nucleotidyltransferase/poly(A) polymerase (Dechlorosoma suillum PS)
MQIYVVGGAVRDRLLGLPVQDRDYVVVGADPQTMLAQGFKPVGKDFPVFLHPQTHEEYAL
ARTERKTGRGYAGFAFHAAPDVTLEQDLARRDLTINAIAEGEDGSLTDPYGGVRDIAARV
LRHVGPAFVEDPVRILRLARFSARFTDFSVAPETLALMREMVANGEVDALVPERVWQELS
KGLMEARPSRMFAVLKDCGALARLLPELEKLDGVPQRADYHPEIDTFDHVMRVIDVAASR
GEPLPVRFAALLHDLGKGTTPADILPRHIGHEHRSVELGAALCARLKVPVECRDLALLTA
RYHGDIHKCLEMRPATLVTLLEKTDAFRRPERFEQLLAACACDYNGRKDWEERPYASPAR
LRTALAAARSVDAGAVAKAAGDRSRIPDAIHQARAGAVAAALRENG