Protein Info for Dsui_0533 in Dechlorosoma suillum PS

Annotation: cobyrinic acid a,c-diamide synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details transmembrane" amino acids 112 to 132 (21 residues), see Phobius details PF01656: CbiA" amino acids 10 to 192 (183 residues), 49.5 bits, see alignment E=6.3e-17 PF07685: GATase_3" amino acids 244 to 424 (181 residues), 107.1 bits, see alignment E=1.4e-34

Best Hits

Swiss-Prot: 59% identical to COBB_PSEU5: Hydrogenobyrinate a,c-diamide synthase (cobB) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K02224, cobyrinic acid a,c-diamide synthase [EC: 6.3.1.- 6.3.5.9] (inferred from 66% identity to eba:ebA4027)

Predicted SEED Role

"Cobyrinic acid A,C-diamide synthase" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.- or 6.3.5.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QFD6 at UniProt or InterPro

Protein Sequence (435 amino acids)

>Dsui_0533 cobyrinic acid a,c-diamide synthase (Dechlorosoma suillum PS)
MTSLACPALLVAAPASGQGKTTVVAALARLHARQGRRVKVFKCGPDFLDPQIHQVASGQP
CDNIDFRMGGEADARWRLARAAAENDLILVEGVMGLNDGEPSAAALARLLGLPILLVLDA
SAMAGSFGAVAFGLKHYREEQGGGAPIGAVLANRVGSAYHAQLCRDTLDEADIAWYGHLP
RDNDAAMPERHLGLLPAAEIEDLKQRLDAMADALAATAAAQLPPPVHFAPAPPPALPPLL
AGKTVAVARDAAFCFAYPANLECLENLGARLVHFSPLADATLPPCDALWLPGGYPELHGA
AIAANRAMAAALQAHAAAGKPLLAECGGMMSLFETLVSVTGESTAGFGLLPGESRMQKKL
AALGLMAAELPEGRLPGHSFHYSTSSTPLEPLVRALKLTGNTPGEPIYRVGRLTASYVHF
YFPANPEAVARLLGA