Protein Info for Dsui_0516 in Dechlorosoma suillum PS

Updated annotation (from data): Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3)
Rationale: Specifically important for utilizing Sodium propionate. Automated validation from mutant phenotype: the predicted function (PROPIONYL-COA-CARBOXY-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: acetyl-CoA carboxylase, biotin carboxylase subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 1 to 108 (108 residues), 155.2 bits, E=2.4e-49
• TIGR00514: acetyl-CoA carboxylase, biotin carboxylase subunit
  • amino acids 1 to 447 (447 residues), 632.1 bits, E=2.6e-194
• PF02786: CPSase_L_D2
  • amino acids 115 to 323 (209 residues), 261.8 bits, E=1.3e-81
• PF07478: Dala_Dala_lig_C
  • amino acids 143 to 292 (150 residues), 32.8 bits, E=1.5e-11
• PF02785: Biotin_carb_C
  • amino acids 337 to 445 (109 residues), 128.4 bits, E=3.7e-41
• PF18140: PCC_BT
  • amino acids 465 to 588 (124 residues), 130.7 bits, E=1.1e-41
• PF00364: Biotin_lipoyl
  • amino acids 599 to 661 (63 residues), 55.2 bits, E=1.6e-18

Best Hits

KEGG orthology group: K01965, propionyl-CoA carboxylase alpha chain [EC: 6.4.1.3] (inferred from 89% identity to dar:Daro_4003)

MetaCyc: 60% identical to propionyl-CoA carboxylase alpha subunit (Cereibacter sphaeroides)
Propionyl-CoA carboxylase. [EC: 6.4.1.3]

Predicted SEED Role

"Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3)" in subsystem Propionyl-CoA to Succinyl-CoA Module (EC 6.4.1.3)

MetaCyc Pathways

• superpathway of anaerobic energy metabolism (invertebrates) (14/17 steps found)
• propanoyl CoA degradation I (3/3 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• superpathway of L-methionine salvage and degradation (9/16 steps found)
• methylaspartate cycle (11/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)
• mycolate biosynthesis (15/205 steps found)
• superpathway of mycolate biosynthesis (16/239 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.3

Use Curated BLAST to search for 6.4.1.3

Sequence Analysis Tools

See G8QFC0 at UniProt or InterPro

Protein Sequence (663 amino acids)

>Dsui_0516 Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3) (Dechlorosoma suillum PS)
MFKKILIANRGEIACRVIKTARKMGIKTVAVYSEADKDALFVEMADEAVCIGPAASKESY
LVADKIIAACKQTGAEAVHPGYGFLSENAEFSRRLEEEGIKFIGPKHYSIAKMGDKIESK
KLAIEAKVNTIPGYNDAIDGPDAAVEIAKKIGYPVMIKASAGGGGKGLRVAYNDAEAHEG
FSSCVNEARNSFGDDRVFIEKYVLEPRHIEIQVLGDSHGNYVYLNERDCSIQRRHQKVIE
EAPSPFVDPEMRKAMGEQAVALARAVNYESAGTVEFVVSGATKEFYFLEMNTRLQVEHPV
TELITGLDLVEQMIRVAYGEKLPLTQADVQINGWAMECRINAEDPFRGFLPSTGRLVKFQ
PPAEVDGQVRVDTGVYDGGEISMYYDSMIAKLIVHGASREQAIARMRDALNGFVIRGISS
NIPFQAALMQHARFQSGIFDTGFIAKEYPKGFDASMVPHDDPALLIGVAAFVHRRYIDRA
AQVSGQLPGHERKVGDEWVVIRNGERHPVVAKPIEGGYLVTYNGEKYELLSDWRQGQSLF
NGTCNGEEFTLQVERHRMTYQLFHWGTRADMMVMSARAAELLALMPEKAAPDLSKFLLSP
MPGLLREVSVAVGQEVKAGEKLAVIEAMKMENILKAEQDCKVKKISVTAGSSLSVDEIII
EFE