Protein Info for Dsui_0422 in Dechlorosoma suillum PS

Annotation: thiamine biosynthesis protein ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 21 to 98 (78 residues), 121.3 bits, E=1.2e-39
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 138 to 603 (466 residues), 710.4 bits, E=3.5e-218
• PF01964: ThiC_Rad_SAM
  • amino acids 140 to 599 (460 residues), 605 bits, E=6.8e-186

Best Hits

Swiss-Prot: 90% identical to THIC_DECAR: Phosphomethylpyrimidine synthase (thiC) from Dechloromonas aromatica (strain RCB)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 90% identity to eba:ebA1712)

MetaCyc: 71% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis II (8/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (7/10 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

See G8QPI0 at UniProt or InterPro

Protein Sequence (638 amino acids)

>Dsui_0422 thiamine biosynthesis protein ThiC (Dechlorosoma suillum PS)
MNANDKFIATAAQVDEAAVQPLPNSRKIYVEGSRPDIRVPMREISQADTPTGFGGEKNPP
IYVYDCSGPYTDPNAKIDIRSGLPALRQQWIEERGDTEVLPDLTSEYGRQRAADPALDEL
RFPGLHRKPRRAKAGANVSQMHYARKGIITPEMEYVAIRENLNRQQYLENLRNTGPMGEK
MVKLLGRQHKGQDFGAAIPQEITPEFVRSEVARGRAIIPNNINHPESEPMIIGRNFLVKI
NANIGNSALGSSISEEVDKMTWSTRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPI
YQALEKVNGKAEELTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYIPMTANRMTGIVSRGG
SIMAKWCLAHHKESFLYTHFEDICEIMKAYDVAFSLGDGLRPGSIYDANDEAQLGELETL
GELTDIAWKHDVQTIIEGPGHVPMHMIKENMDLQLKHCKEAPFYTLGPLTTDIAPGYDHI
TSGIGAAMIGWYGTAMLCYVTPKEHLGLPDKNDVKEGIITYKLAAHAADLAKGHPGAQIR
DNALSKARYEFRWDDQFNLGLDPDKAKSFHDETLPKESAKVAHFCSMCGPHFCSMKITQD
VRDFAAQQGIGEAEALEKGMETKAVEFVKSGAELYRKV