Protein Info for Dsui_0401 in Dechlorosoma suillum PS

Annotation: nucleoside-diphosphate-sugar epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 1 to 240 (240 residues), 65.9 bits, E=1.3e-21
• PF00106: adh_short
  • amino acids 3 to 110 (108 residues), 29.1 bits, E=2.6e-10
• PF01370: Epimerase
  • amino acids 3 to 238 (236 residues), 195.1 bits, E=5.2e-61
• PF02719: Polysacc_synt_2
  • amino acids 3 to 231 (229 residues), 43.3 bits, E=1.1e-14
• PF01073: 3Beta_HSD
  • amino acids 4 to 229 (226 residues), 61.1 bits, E=3.6e-20
• PF16363: GDP_Man_Dehyd
  • amino acids 4 to 322 (319 residues), 169.3 bits, E=6.4e-53
• PF07993: NAD_binding_4
  • amino acids 5 to 166 (162 residues), 24.9 bits, E=4.2e-09

Best Hits

Swiss-Prot: 57% identical to CAPI_STAAU: Protein CapI (capI) from Staphylococcus aureus

KEGG orthology group: K01795, [EC: 5.1.3.-] (inferred from 78% identity to eba:ebA1639)

MetaCyc: 52% identical to UDP-glucuronate 4-epimerase (Streptococcus pneumoniae)
UDP-glucuronate 4-epimerase. [EC: 5.1.3.6]

Predicted SEED Role

"dTDP-glucose 4,6-dehydratase (EC 4.2.1.46)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 4.2.1.46)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• dTDP-β-L-rhamnose biosynthesis (5/5 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (5/6 steps found)
• UDP-α-D-galacturonate biosynthesis I (from UDP-D-glucuronate) (1/1 steps found)
• dTDP-3-acetamido-α-D-fucose biosynthesis (3/5 steps found)
• dTDP-4-O-demethyl-β-L-noviose biosynthesis (3/5 steps found)
• dTDP-β-D-fucofuranose biosynthesis (2/4 steps found)
• dTDP-6-deoxy-α-D-allose biosynthesis (2/4 steps found)
• dTDP-N-acetylthomosamine biosynthesis (2/4 steps found)
• dTDP-N-acetylviosamine biosynthesis (2/4 steps found)
• dTDP-α-D-ravidosamine and dTDP-4-acetyl-α-D-ravidosamine biosynthesis (3/6 steps found)
• dTDP-D-desosamine biosynthesis (3/6 steps found)
• dTDP-L-daunosamine biosynthesis (3/6 steps found)
• dTDP-sibirosamine biosynthesis (3/6 steps found)
• dTDP-α-D-mycaminose biosynthesis (2/5 steps found)
• dTDP-3-acetamido-3,6-dideoxy-α-D-glucose biosynthesis (2/5 steps found)
• dTDP-β-L-digitoxose biosynthesis (3/7 steps found)
• dTDP-β-L-olivose biosynthesis (3/7 steps found)
• dTDP-β-L-4-epi-vancosamine biosynthesis (3/8 steps found)
• dTDP-β-L-megosamine biosynthesis (3/8 steps found)
• L-ascorbate biosynthesis V (euglena, D-galacturonate pathway) (2/7 steps found)
• dTDP-β-L-mycarose biosynthesis (2/7 steps found)
• UDP-sugars interconversion (3/9 steps found)
• dTDP-α-D-forosamine biosynthesis (3/9 steps found)
• dTDP-α-D-olivose, dTDP-α-D-oliose and dTDP-α-D-mycarose biosynthesis (3/9 steps found)
• superpathway of enterobacterial common antigen biosynthesis (2/10 steps found)
• superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis (6/19 steps found)
• superpathway of novobiocin biosynthesis (4/19 steps found)
• superpathway of erythromycin biosynthesis (3/19 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• superpathway of megalomicin A biosynthesis (4/22 steps found)
• superpathway of dTDP-glucose-derived antibiotic building blocks biosynthesis (3/23 steps found)

KEGG Metabolic Maps

• Biosynthesis of vancomycin group antibiotics
• Nucleotide sugars metabolism
• Polyketide sugar unit biosynthesis
• Starch and sucrose metabolism
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.46

Use Curated BLAST to search for 4.2.1.46 or 5.1.3.- or 5.1.3.6

Sequence Analysis Tools

See G8QP16 at UniProt or InterPro

Protein Sequence (334 amino acids)

>Dsui_0401 nucleoside-diphosphate-sugar epimerase (Dechlorosoma suillum PS)
MKILVTGAAGFIGMHTAQRLLERGDEVVGVDNLNDYYEVSLKEARLARLAAYPNFRFVRL
DIADSQGMQELFAAEGFQRVVHLAAQAGVRYSLANPHAYAQSNLVGFVNVLEGCRHAQAE
HLVYASSSSVYGGNRKMPFSEHDAVDHPVSLYAATKKSNELMAHTYSHLFGLPTTGLRFF
TVYGPWGRPDMALFLFTRAILEGRPIDVFNHGKMQRDFTYIDDIVEGVVRVLDQPARASE
SFDGLAPDPACSNVPYRLFNIGNDQPVDLLAFIEALEGALGQVANKRFLPLQDGDVPATW
ADTALLGQAVGFAPKTSVQDGVGQFVRWYRDYYR