Protein Info for Dsui_0366 in Dechlorosoma suillum PS

Annotation: DNA-directed RNA polymerase, beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1427 signal peptide" amino acids 1 to 35 (35 residues), see Phobius details TIGR02013: DNA-directed RNA polymerase, beta subunit" amino acids 8 to 1423 (1416 residues), 1830.1 bits, see alignment E=0 PF04563: RNA_pol_Rpb2_1" amino acids 27 to 574 (548 residues), 95.4 bits, see alignment E=1.1e-30 PF04561: RNA_pol_Rpb2_2" amino acids 154 to 233 (80 residues), 28.4 bits, see alignment 3.7e-10 amino acids 482 to 529 (48 residues), 33.7 bits, see alignment (E = 9.3e-12) PF04565: RNA_pol_Rpb2_3" amino acids 588 to 655 (68 residues), 105.9 bits, see alignment 3e-34 PF10385: RNA_pol_Rpb2_45" amino acids 666 to 731 (66 residues), 92.5 bits, see alignment 4.1e-30 PF00562: RNA_pol_Rpb2_6" amino acids 795 to 1349 (555 residues), 446.2 bits, see alignment E=3.2e-137 PF04560: RNA_pol_Rpb2_7" amino acids 1351 to 1424 (74 residues), 96.5 bits, see alignment 3.1e-31

Best Hits

Predicted SEED Role

"DNA-directed RNA polymerase beta subunit (EC 2.7.7.6)" in subsystem RNA polymerase bacterial (EC 2.7.7.6)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.6

Use Curated BLAST to search for 2.7.7.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QNY5 at UniProt or InterPro

Protein Sequence (1427 amino acids)

>Dsui_0366 DNA-directed RNA polymerase, beta subunit (Dechlorosoma suillum PS)
MTYSYTEKKRIRKSFAKRASVLDVPFLLATQLESYTAFLQAELPTAKRRNQGLQAAFGSI
FPIVSHSGNARLEFVSYQLAEPAFDVKECQQRGLTFASALRAKVRLVIMDREAPDTVKEV
KEQEVYMGEIPLMTTTGSFVINGTERVIVSQLHRSPGVFFEHDRGKTHSSGKLLFSARVI
PYRGSWLDFEFDPKDILFFRVDRRRKMPVTTLLKAIGLTNEQILAGFFEFDDFKLLKNGA
DFTLVPERLRGETARFDFCDKAGKVIVPKDKRITAKHIRDLDAAGIKHIAVPDDFLVGRV
IGHNIIDKDSGEVIANANDEITESLLAKLREAGVAEFQTLYTNDLDRGAFISNTLRADDT
ATKQAARVAIYRMMRPGEPPTEEAVEILFNGLFYSDDRYDLSGVGRMKFNRRIGRPDVVE
YKVTIKNAPHKTEALAKAIAEFTGANQGTVQEILNELQYGSRAAAENLTEEAAHTLAAQL
KALGANVEVREQLTLSPRDIVESIKLLVELRNGRGEIDDIDHLGNRRVRSVGELAENQFR
AGLVRVERAVKERLSQAESDNLMPHDLINAKPISAAIKEFFGSSQLSQFMDQTNPLSEIT
HKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLALFARTNSYG
FLETPYRKVVDGKVTDQIEYLSAIEEGAYVVAQANAALGADGTLVDELVTCREKGETILA
EPSRVQYMDVAPGQIVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPLVGTGIE
RTVAVDSGTAVQALRGGVVDYVDASRVVVRVHDDETVPGEVGVDIYNLTKYTRSNQNTNI
NQRPLVKMGDIIAKGDVVADGASTDKGELALGQNMLIAFMPWNGYNFEDSILISERVVAE
DRYTSIHIEELTVVARDTKLGPEEITRDIASLGEVQLSRLDESGVVYIGAEVEAGDVLVG
KVTPKGETQLTPEEKLLRAIFGEKASDVKDTSLRVPSGIAGTVIDVQVFTREGIERDKRA
QSIIDDHLRGYKQDLADQMRIVERDTFARVGRLITGKPANGGPKKLAKGTVVTQEYLDGI
DPHNWFDVRMADEEVAQQLEQLKESLEQTRKDFDIAFEEKRKKLTQGDELPPGVQKMVKV
YVAVKRRLQPGDKMAGRHGNKGVVSRIVPVEDMPYMADGRPVDIVLNPLGVPSRMNVGQI
LEVHLGWAAKGLGYKIGDMLQAGAKAAEVRKFLEEIYNSEGKKENLNELNDQEIMELAGN
LTTGVPFATPVFDGADEAEIKAMLRLAGMPESGQITLHDGRTGDAFERQVTVGYMHYLKL
HHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASYVLQEMLTVKS
DDVNGRTKVYENIVKGEHKIDAGMPESFNVLVKEIRSLAIDIDLERY