Protein Info for Dsui_0294 in Dechlorosoma suillum PS

Annotation: D-alanyl-D-alanine carboxypeptidase, serine-type, PBP4 family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 503 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details PF02113: Peptidase_S13" amino acids 56 to 469 (414 residues), 269.2 bits, see alignment E=6.3e-84 TIGR00666: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase" amino acids 77 to 466 (390 residues), 185.7 bits, see alignment E=6e-59

Best Hits

KEGG orthology group: K07259, D-alanyl-D-alanine carboxypeptidase / D-alanyl-D-alanine-endopeptidase (penicillin-binding protein 4) [EC: 3.4.16.4 3.4.21.-] (inferred from 52% identity to dar:Daro_0423)

Predicted SEED Role

"D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4)" in subsystem Peptidoglycan Biosynthesis (EC 3.4.16.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.16.4, 3.4.21.-

Use Curated BLAST to search for 3.4.16.4 or 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QNB9 at UniProt or InterPro

Protein Sequence (503 amino acids)

>Dsui_0294 D-alanyl-D-alanine carboxypeptidase, serine-type, PBP4 family (Dechlorosoma suillum PS)
MRIFHRSPSATGRLALALWLTLLCNPLHTYAAGQMPPDTATVLPPEVTRALNAAGLPPGQ
FAVAVQAVDAAAPRLLHNAARPMNPASVMKLVTTYAALEKLGPAYTWKTELRGDNAPRDG
RLEGNLYLVGSGDPALTLERFWLLLRQLRDRAEVRQIGGDLVADRRAYRLPPFDPAAFDN
EPLRAYNVNADAALVNYTALRFTLVPDLAAGSVRLVQEVPQEGLAVNARIAPGAGECGDW
REKLTVRFDPTGKRLDIGGSFPLVCGEKPLHLAPLAGDAYLAGLFRPLWRELGGQFAGQV
REGPAPVGGVPLLRWESPSLAEIVRDTNKYSSNVMARHLFLALADTPAGEAATLAGARQG
LRQVLAEKGLSFPELAVENGSGLSRDDRIAARSLLQLLLAAWKSPVMPEFVASLPLAGRD
GTLKRRFGNGSSAGRAHLKTGSIEGVRAWAGYVTDAGGRRWALVALAGGPKAGAAWGPVE
ALLEWVATQSPTPLAAPGTPLSN