Protein Info for CSW01_19450 in Vibrio cholerae E7946 ATCC 55056

Annotation: bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1039 transmembrane" amino acids 716 to 719 (4 residues), see Phobius details PF14850: Pro_dh-DNA_bdg" amino acids 73 to 187 (115 residues), 148.8 bits, see alignment E=1.3e-47 PF01619: Pro_dh" amino acids 196 to 490 (295 residues), 340.6 bits, see alignment E=1.3e-105 TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase" amino acids 525 to 1038 (514 residues), 930.1 bits, see alignment E=1.3e-284 PF00171: Aldedh" amino acids 580 to 1037 (458 residues), 387.9 bits, see alignment E=8.8e-120

Best Hits

KEGG orthology group: K13821, proline dehydrogenase / delta 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12 1.5.99.8] (inferred from 70% identity to vsa:VSAL_II0819)

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.12 or 1.5.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1039 amino acids)

>CSW01_19450 bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA (Vibrio cholerae E7946 ATCC 55056)
MFTATDVLNAAFIEQPLDKLWSLISPLYMVDESQWLTQLLPLATPSHAEKAAMAEKTTRL
IEAIRSDKKAVQMIDALLLEYSLDTQEGILLMCLAEALMRIPDAETADAFIKDRLGVADW
KSHLKNSDSVFVNASTWGLMLTGKVIGLADGDTASPVQAVNRLVNKLTEPVIRKAMHQAM
KIMGHQFVLGRTIEEAQKNGRPMRDKGYTYSFDMLGEAALTSADAHKYFKDYLMAIEAVG
RDKYGLETSPAPSVSIKLSALHPRYQVANADRVMTELYSTLIQLLERAKELDVAITIDAE
EADRLELSLHLFEKLYRSDTLRGWGKFGLVVQAYSKRALPVLVWLTALAKEQGDLIPVRL
VKGAYWDSEIKMSQQRGFTGYPVYTRKEATDVSYLACARFLLSESVRGNLFPQFASHNAQ
TVTAIAVMAQHKDFEFQRLHGMGDALYHHAKATYQQSVRIYAPVGSHKDLLPYLVRRLLE
NGANSSFVHRLVDSRCPVGALTQHPVDMLLAFETLNNRKIPLPTEIFAERKNSLGINIDI
ESEAKPFEAQIHAWLDKTWQAAPIIGGHSYYESMIKADHSAEPVTAPYDRRIQIGQVFHA
NLDHVSAAIDSAQQAFATWNALDAKERASKLDALADLLELHMPELVALCHQEAGKTIHDS
IDEVREAVDFCRYYGKQVDVLGEFSVESFDGSTRRVSRQGRGVFVCISPWNFPLAIFLGQ
ISAALVAGNTVIAKPAEQTSLIAYRAVELMQEAGFPAGTIQLLPGRGADIGSALTSHPAI
AGVAFTGSTATAQRINQTLAQREAAPVPFIAETGGQNAMIVDSTALPEQVVRDVLRSAFA
SAGQRCSALRVLFVQQDIADRVITLIQGAMQELKVSVPHLHQTDVGPVIDEKAKQKLLAH
IEHMSQTQKKIAQLTLDDACQHGDFVAPTAFEIDDIAALSEEQFGPILHIVRFKARELAQ
IVDKINQTGFGLTMGIHSRNETTYRWIEKHARVGNCYINRDQVGAVVGVQPFGGQGLSGT
GPKAGGPHYLYRFTQVQYQ