Protein Info for CSW01_18780 in Vibrio cholerae E7946 ATCC 55056

Annotation: 23S rRNA (uracil(747)-C(5))-methyltransferase RlmC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02085: 23S rRNA (uracil-5-)-methyltransferase RumB
  • amino acids 2 to 374 (373 residues), 701.9 bits, E=9.4e-216
• PF05958: tRNA_U5-meth_tr
  • amino acids 208 to 374 (167 residues), 65.1 bits, E=1.2e-21
• PF05175: MTS
  • amino acids 235 to 311 (77 residues), 26 bits, E=1.3e-09
• PF13847: Methyltransf_31
  • amino acids 235 to 296 (62 residues), 33.3 bits, E=7.7e-12
• PF09445: Methyltransf_15
  • amino acids 237 to 313 (77 residues), 23.2 bits, E=9.2e-09

Best Hits

Swiss-Prot: 100% identical to RLMC_VIBCH: 23S rRNA (uracil(747)-C(5))-methyltransferase RlmC (rlmC) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K03212, RNA methyltransferase, TrmA family [EC: 2.1.1.-] (inferred from 100% identity to vch:VCA0929)

MetaCyc: 55% identical to 23S rRNA m5U747 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11600 [EC: 2.1.1.189]

Predicted SEED Role

"23S rRNA (Uracil-5-) -methyltransferase rumB (EC 2.1.1.-)" (EC 2.1.1.-)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.189

Protein Sequence (375 amino acids)

>CSW01_18780 23S rRNA (uracil(747)-C(5))-methyltransferase RlmC (Vibrio cholerae E7946 ATCC 55056)
MQCEFFIQKRCTSCHLCAQPYSEQVTNKDQQLRELIAPATDVQWLPPVTSADTAFRNKAK
MVVLGAAHAPILGIEDAQGQPLSLVTCPLYPQPMQELLAYLENWIRIAGIPPYNKLKKKG
ELKFILLTRSENSGQFMLRFVARSHAVLERIERNLPTLIAAFPMIEVISVNIQPVHMARL
EGEEEIFLTETQSLLEHFNDVPMVIRPKSFFQTNPKVAEQLYATARTWVREIAPTQMWDL
FCGVGGFALHCAAPDTAVTGIEIEPEAIASAQRSAQMMGIDNLSFAALDSAKFSQSQMSA
PELVLVNPPRRGLGSELTAQLEALAPKYILYSSCNPQTMVKDIAELSSYQMIRVQWFDMF
PHTDHAEVLTLLVRK