Protein Info for CSW01_18275 in Vibrio cholerae E7946 ATCC 55056

Annotation: diaminobutyrate--2-oxoglutarate transaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 421 TIGR00709: 2,4-diaminobutyrate 4-transaminase" amino acids 2 to 410 (409 residues), 470.4 bits, see alignment E=4.7e-145 TIGR02407: diaminobutyrate--2-oxoglutarate aminotransferase" amino acids 2 to 409 (408 residues), 650.2 bits, see alignment E=1.1e-199 PF00202: Aminotran_3" amino acids 16 to 407 (392 residues), 251.8 bits, see alignment E=5.2e-79

Best Hits

Swiss-Prot: 100% identical to ECTB_VIBCH: Diaminobutyrate--2-oxoglutarate transaminase (ectB) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K00836, diaminobutyrate-2-oxoglutarate transaminase [EC: 2.6.1.76] (inferred from 100% identity to vcj:VCD_000500)

Predicted SEED Role

"Diaminobutyrate-pyruvate aminotransferase (EC 2.6.1.46)" in subsystem Ectoine biosynthesis and regulation (EC 2.6.1.46)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.76

Use Curated BLAST to search for 2.6.1.46 or 2.6.1.76

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (421 amino acids)

>CSW01_18275 diaminobutyrate--2-oxoglutarate transaminase (Vibrio cholerae E7946 ATCC 55056)
MDIFKHHESQVQSYANHFPVLFGTAKGSWLYSQQGDAYLDFLSGAGALNYGHNNAVLKQA
LLEYIERDGLTHGLDMHSEAKAHFIQALQTHILEPRGLNYKLQFTGPTGTNAVEAALKLA
RKVTGRHNVVTFTNGFHGCSLGALAATGNQHHRQGAGLALSGVYRVPYDGYAGVDGLTLF
ETMLQDNSSGLDKPAAVLLETVQGEGGLNVASDAWLQRVQAICRAQQILLIVDDIQAGCG
RTGTFFSFEPSGIEPDMVTLSKSLSGYGLPMALVLFKPEWDQWKPGEHNGTFRGNNHAFV
TATRALEAYWANQDFQTHIAARSEQVTQALLQCLSRYPTLFSGLKGRGLMQGLACHNGDI
ARDIAALCFQKGLIIETAGAEDEVLKVFCPLTITEADLAHGLTIIERSAARIAPRGLQQA
S