Protein Info for CSW01_17040 in Vibrio cholerae E7946 ATCC 55056

Annotation: NAD(P)(+) transhydrogenase (Re/Si-specific) subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 512 transmembrane" amino acids 168 to 191 (24 residues), see Phobius details amino acids 401 to 419 (19 residues), see Phobius details amino acids 425 to 444 (20 residues), see Phobius details amino acids 454 to 473 (20 residues), see Phobius details amino acids 479 to 501 (23 residues), see Phobius details TIGR00561: NAD(P)(+) transhydrogenase (AB-specific), alpha subunit" amino acids 2 to 512 (511 residues), 907.6 bits, see alignment E=1.1e-277 PF05222: AlaDh_PNT_N" amino acids 4 to 137 (134 residues), 160.7 bits, see alignment E=3.5e-51 PF01262: AlaDh_PNT_C" amino acids 141 to 368 (228 residues), 233.9 bits, see alignment E=2.1e-73 PF12769: PNTB_4TM" amino acids 428 to 511 (84 residues), 124.5 bits, see alignment E=2.9e-40

Best Hits

Swiss-Prot: 77% identical to PNTA_ECOLI: NAD(P) transhydrogenase subunit alpha (pntA) from Escherichia coli (strain K12)

KEGG orthology group: K00324, NAD(P) transhydrogenase subunit alpha [EC: 1.6.1.2] (inferred from 100% identity to vco:VC0395_0497)

MetaCyc: 77% identical to pyridine nucleotide transhydrogenase subunit alpha (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-277 [EC: 7.1.1.1]

Predicted SEED Role

"NAD(P) transhydrogenase alpha subunit (EC 1.6.1.2)" in subsystem Phosphate metabolism (EC 1.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.1.2

Use Curated BLAST to search for 1.6.1.2 or 7.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (512 amino acids)

>CSW01_17040 NAD(P)(+) transhydrogenase (Re/Si-specific) subunit alpha (Vibrio cholerae E7946 ATCC 55056)
MQIGVPREQLAGETRVAASPSSVEQLIKLGFEVCIESKAGALASFDDAAYTAAGATIGSR
EEIWACPLILKVNAPSDDEIALLKEGATLVSFIWPAQNPALMEKLSSKNINVLAMDAVPR
ISRAQALDALSSMANIAGYRAVVEAAHEFGRFFTGQITAAGKVSPAKVFVAGAGVAGLAA
IGAAGSLGAIVRAFDVRPEVKEQVQSMGAEFLEVNFQETAGSGDGYAKEMSDEFNRKAAE
LYAAQAKDVDIIITTALIPGKPAPKLITKEMVDSMKAGSVIVDLAAANGGNCEYTVKDQV
ITTDNGVKVIGYTDMVGRLPTQSSQLYATNLVNLLKLLCKEKDGNIDINFEDVVLRGVTV
VKAGEITWPAPPIQVSAQPQQKAKVQPTKAQKKEPEPTSPVKKLVGLAVGVGLFAWVASV
APAAFLGHFTVFVLACVVGYYVVWNVTHALHTPLMSVTNAISGIIVVGALLQIGQGSGIV
TFLAFIAVLIASINIFGGFTVTKRMLEMFRKN