Protein Info for CSW01_16800 in Vibrio cholerae E7946 ATCC 55056

Annotation: anaerobic ribonucleoside-triphosphate reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03477: ATP-cone
  • amino acids 4 to 89 (86 residues), 79.8 bits, E=2.6e-26
• TIGR02487: anaerobic ribonucleoside-triphosphate reductase
  • amino acids 115 to 705 (591 residues), 659.3 bits, E=2.6e-202
• PF13597: NRDD
  • amino acids 116 to 705 (590 residues), 635.5 bits, E=1.3e-194
• PF01228: Gly_radical
  • amino acids 582 to 686 (105 residues), 103.2 bits, E=1.6e-33

Best Hits

Swiss-Prot: 77% identical to NRDD_ECOLI: Anaerobic ribonucleoside-triphosphate reductase (nrdD) from Escherichia coli (strain K12)

KEGG orthology group: K00527, ribonucleoside-triphosphate reductase [EC: 1.17.4.2] (inferred from 80% identity to vsa:VSAL_II0791)

MetaCyc: 77% identical to anaerobic ribonucleoside-triphosphate reductase (Escherichia coli K-12 substr. MG1655)
RIBONUCLEOSIDE-TRIP-REDUCT-RXN [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]

Predicted SEED Role

"Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2)" in subsystem Ribonucleotide reduction (EC 1.17.4.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (8/8 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (7/7 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (5/7 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.98.6 or 1.17.4.2

Protein Sequence (706 amino acids)

>CSW01_16800 anaerobic ribonucleoside-triphosphate reductase (Vibrio cholerae E7946 ATCC 55056)
MKPIVIKRDGSKAPFNRDRIQAAVESAAEHADQEIAIYALNVALAVELKLRDYDEVHITE
IQTLVENELMQGPYKALARSYIEYRHDRDVAREKQSKLTKEIEGLIQESNADLLNENANK
DGKVIPTQRDLLAGIVAKHYAKTRILPRDVVQAHESGDIHYHDLDYAPFFPMFNCMLIDL
KGMLTHGFKMGNAEIDTPKSISTATAVTAQIIAQVASHIYGGTTINRIDEVLAPYVTASY
EKHLEIAREWDIHSPEAFAKARTEKECYDAFQSLEYEVNTLHTANGQTPFVTFGFGLGTS
WESRLIQQSILKNRIAGLGKNRKTAVFPKLVFAIKDGLNHKTEDPNYDIKQLALECASKR
MYPDILNYDKVVEVTGSFKTPMGCRSFLNPYEENGELIHEGRNNLGVVSLNLPRIALQAK
GDINKFYALLDDKLKLARRALDTRINRLENVKARVAPILYMEGACGVRLKANDSIADIFK
HGRASISLGYIGVHETIMALFGQQKHVYDDVQLREEAVKIIQHLRNAVEQWKKETGYAFS
LYGTPSENLCSRFCRIDAKQFGVVEGVTDKGYYTNSFHLDVQKKVNPYDKIDFEMPYPEI
SSGGFICYGEFPNMQRNVEALENVWDYSYHRVPYYGTNTPIDECYECGFTGEFDCTSKGF
VCPRCGNHEPTKVSVTRRVCGYLGSPDARPFNVGKQEEVKRRVKHL