Protein Info for CSW01_14125 in Vibrio cholerae E7946 ATCC 55056

Annotation: 1,4-alpha-glucan branching enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 729 transmembrane" amino acids 141 to 160 (20 residues), see Phobius details PF22019: GlgB_N" amino acids 15 to 100 (86 residues), 38.8 bits, see alignment E=1.6e-13 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 116 to 723 (608 residues), 812.5 bits, see alignment E=1.2e-248 PF02922: CBM_48" amino acids 134 to 208 (75 residues), 70.9 bits, see alignment E=1.9e-23 PF00128: Alpha-amylase" amino acids 276 to 348 (73 residues), 26.5 bits, see alignment E=7.6e-10 PF02806: Alpha-amylase_C" amino acids 629 to 726 (98 residues), 71.1 bits, see alignment E=1.6e-23

Best Hits

Swiss-Prot: 100% identical to GLGB_VIBCH: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 100% identity to vco:VC0395_0117)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.18

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (729 amino acids)

>CSW01_14125 1,4-alpha-glucan branching enzyme (Vibrio cholerae E7946 ATCC 55056)
MKITKKPSKVQQFYDQLARAAFADPFSFLGPYIPAEQGALRVWMPGADNVALVVEGQARV
ALEREGEGGFVLKDGRNLRFTHYQLAVDWAGTEQLLDDPYQYHGLYAEYEDLHTPKQMYH
HMGAQFVTLERDGKMVSGVRFLVYAPHAAACSLIGAFNHWDGRRHPMQRLDYGIWGIFIP
GLPEGTQYKFELKGPHGEGLPHKADPWGFYAEQYPSFASVTYDHRRYQWQDTAWQQRPVT
EKRKQALSFYELHVGSWKRGENGEFLNYRELADQLVPYLVEMGYTHVELMPVAEHPFYGS
WGYQPVGLFAPTSRYGSPDDFKYFVDLCHQAGIGVVLDWVPAHFPSDSHGLANFDGTPLF
HDPDPRRGWHQDWNSYIYDLGREHVRRFLVANALYWFEMFHIDGIRVDAVASMLYLDYSR
SHDQWIPNVDGGRENYDAIATFKWMNEEVYKHFPNAMTIAEESTAFPGVSAPTFMGGLGF
GFKWNMGWMHDSLSYIKEDPVHRKYHHNTLTFPLIYAFSENYVLSLSHDEVVYGKRSLMY
KMPGDEWQQTANLRAYLGYMYGQPGKKLNFMGTELGQTAEWDHDGQLQWFLTQFERHAGI
QRLVRDLNHLYQAQTALHQLDCDPRGFEWRLQDNADLSVIAHERMDEAGNRVLVITNFTP
VPQQEFRLGVPKTGKYRLLLNTDAKQYNGSDYPVLQDVSTEAISSEGLDQSLLLSVPPLA
TLFYQWSAK