Protein Info for CSW01_14115 in Vibrio cholerae E7946 ATCC 55056

Annotation: glycogen/starch/alpha-glucan phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 817 TIGR02093: glycogen/starch/alpha-glucan phosphorylases" amino acids 11 to 812 (802 residues), 1218.5 bits, see alignment E=0 PF00343: Phosphorylase" amino acids 99 to 812 (714 residues), 1063.6 bits, see alignment E=0

Best Hits

Swiss-Prot: 51% identical to PHSG_SYNY3: Glycogen phosphorylase (glgP) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K00688, starch phosphorylase [EC: 2.4.1.1] (inferred from 100% identity to vcj:VCD_000219)

MetaCyc: 50% identical to glycogen phosphorylase b subunit (Drosophila melanogaster)
Phosphorylase. [EC: 2.4.1.1]

Predicted SEED Role

"Glycogen phosphorylase (EC 2.4.1.1)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (817 amino acids)

>CSW01_14115 glycogen/starch/alpha-glucan phosphorylase (Vibrio cholerae E7946 ATCC 55056)
MKPTQQKQFDKKLFQENVKRHLTATYATTVEHASARAWYLAMGRALAEFTTFDLLETEQD
ERILNSKSLNYLSLEFLIGRLTGNNLISMGVYEAVGQAMEELGQNLSDLLEEERDPSLGN
GGLGRLAACFMDSCAAQEYPTVGYGLHYEYGLFKQSFEEGRQKEAPDAWCGVEGYPWEVA
RPELKQEIGFYGHVEVVNENGKERRRWVPGMLVQAMPWDLPIVGYQSDTVYPLRLWECRA
IAPFSLESFNNGNYFEAQHALIDAGNITKVLYPNDNHEKGKTLRLMQQYFHSAASVRDIL
RRHEAAGHALADLPKYETIQLNDTHPTIAIPELMRILIDEKLMSWEAAWAICSHTFAYTN
HTLLPEALETWSESLIQRLLPRHMEIIYEINHRFLQQVRAKWPGDVAKQQKLSIIEEGFH
RMVRMANLCVVGSYAVNGVAALHSELVKRDLFPEFVELYPGKIQNVTNGITPRRWLKFCN
PGLSALISEKIGHEWPAKLDQLTKVAQYAEDAAFQKRFMEVKKANKARLADWVKDHMGIE
LDTNAIFDVQIKRLHEYKRQHLNMLHILSLYHRLINDPSFDMHPRVVFFAAKAAPGYHLA
KEIIYAINMIAQKVNNDPRVGNKLKVVFIPDYRVSMAEIIIPAADVSEQISTAGKEASGT
GNMKMALNGALTIGTMDGANVEIREEVGDDNIYIFGLEVDGVEALKARGYNPYDFYHADP
LLKASLDLLVGEEFTPGAPGKLRATYDSLLDGGDPYLVLADFASYVKAHEAIDKQYRDQA
GWAKKAILNTALVGKFSSDRSIRDYVNNIWKLSAVQR