Protein Info for CSW01_13860 in Vibrio cholerae E7946 ATCC 55056

Annotation: nitrogen regulation protein NR(II)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 354 PF00989: PAS" amino acids 9 to 104 (96 residues), 60.5 bits, see alignment E=2.3e-20 PF00512: HisKA" amino acids 134 to 189 (56 residues), 51.5 bits, see alignment E=1.3e-17 PF02518: HATPase_c" amino acids 236 to 350 (115 residues), 75.4 bits, see alignment E=7.4e-25

Best Hits

Swiss-Prot: 80% identical to NTRB_VIBAL: Sensory histidine kinase/phosphatase NtrB (ntrB) from Vibrio alginolyticus

KEGG orthology group: K07708, two-component system, NtrC family, nitrogen regulation sensor histidine kinase GlnL [EC: 2.7.13.3] (inferred from 100% identity to vcm:VCM66_2668)

Predicted SEED Role

"Nitrogen regulation protein NtrB (EC 2.7.13.3)" (EC 2.7.13.3)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (354 amino acids)

>CSW01_13860 nitrogen regulation protein NR(II) (Vibrio cholerae E7946 ATCC 55056)
MSVSAELSQTIINNQVTSVLILDESLMIRYANPAAEQLFSQSAKRLMHQSLNHLVQHSSL
DLQLLTQPLQSGQSITDSDVTLVIDSKPLMLEVTVSPISWHKELLLLAEMRTIGQQRRLT
QELNQHAQQQAAKLLVRGLAHEIKNPLGGLRGAAQLLERMLPDPALMEYTQIIIEQADRL
RGLVDRLLGPQRPGEKKWENLHLILEKVRQLVELEAGANLVFERDYDPSLPNILMDTDQI
EQALLNIVSNAAQILTNQTHGVITLRTRTVHQANIHGQRHKLVASIEIIDNGPGIPPELQ
DTLFYPMVSGREGGTGLGLSISQNLIDQHQGKIEVQSWPGRTVFTIYLPILNCC