Protein Info for CSW01_13575 in Vibrio cholerae E7946 ATCC 55056

Annotation: ATP-dependent 6-phosphofructokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00365: PFK
  • amino acids 4 to 276 (273 residues), 380.1 bits, E=3.3e-118
• TIGR02482: 6-phosphofructokinase
  • amino acids 4 to 301 (298 residues), 434.3 bits, E=1e-134

Best Hits

Swiss-Prot: 100% identical to PFKA_VIBCH: ATP-dependent 6-phosphofructokinase (pfkA) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K00850, 6-phosphofructokinase [EC: 2.7.1.11] (inferred from 100% identity to vcj:VCD_001679)

MetaCyc: 76% identical to 6-phosphofructokinase 1 (Escherichia coli K-12 substr. MG1655)
6-phosphofructokinase. [EC: 2.7.1.11]; 2.7.1.- [EC: 2.7.1.11]

Predicted SEED Role

"6-phosphofructokinase (EC 2.7.1.11)" in subsystem D-Tagatose and Galactitol Utilization or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or N-Acetyl-Galactosamine and Galactosamine Utilization (EC 2.7.1.11)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of N-acetylneuraminate degradation (20/22 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (16/17 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (17/19 steps found)
• glycolysis I (from glucose 6-phosphate) (12/13 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• glycolysis III (from glucose) (9/11 steps found)
• superpathway of hexitol degradation (bacteria) (14/18 steps found)
• glycolysis IV (8/10 steps found)
• sedoheptulose bisphosphate bypass (2/2 steps found)
• formaldehyde assimilation II (assimilatory RuMP Cycle) (7/9 steps found)
• homolactic fermentation (9/12 steps found)
• D-galactosamine and N-acetyl-D-galactosamine degradation (2/4 steps found)
• 1,3-propanediol biosynthesis (engineered) (5/9 steps found)
• N-acetyl-D-galactosamine degradation (2/5 steps found)
• galactitol degradation (2/5 steps found)
• lactose degradation I (1/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Fructose and mannose metabolism
• Galactose metabolism
• Glycolysis / Gluconeogenesis
• Pentose phosphate pathway

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.11

Protein Sequence (320 amino acids)

>CSW01_13575 ATP-dependent 6-phosphofructokinase (Vibrio cholerae E7946 ATCC 55056)
MIKKIGVLTSGGDAPGMNAAIRGVVRTALGAGLEVFGIYDGYQGLYEDRIKQLDRSSVSD
VINRGGTFLGSARFPQFREVEVREKAIENLKKHGIEALVVIGGDGSYMGAKKLTEMGFPC
IGLPGTIDNDIAGTDYTIGYLTALNTVIEAIDRLRDTSSSHQRISIVEIMGRHCGDLTLT
SAIAGGCEYIITPETGLNMEQLISNIKDGIAKGKKHAIIALTELMMDANKLAKEIESATG
RETRATVLGHIQRGGKPTAFDRVLASRMGNYAVHLLMEGHGGRCVGIQKEQLVHHDIIDA
IENMKRPVRTDLYKVAEELF