Protein Info for CSW01_13255 in Vibrio cholerae E7946 ATCC 55056

Annotation: aminodeoxychorismate/anthranilate synthase component II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00566: glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase
  • amino acids 1 to 192 (192 residues), 286.3 bits, E=5.6e-90
• PF00117: GATase
  • amino acids 3 to 190 (188 residues), 212.9 bits, E=3.4e-67
• PF07722: Peptidase_C26
  • amino acids 67 to 175 (109 residues), 38.6 bits, E=1e-13

Best Hits

Swiss-Prot: 76% identical to TRPG_PSEAE: Anthranilate synthase component 2 (trpG) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01664, para-aminobenzoate synthetase component II [EC: 2.6.1.85] (inferred from 100% identity to vcm:VCM66_2539)

MetaCyc: 76% identical to anthranilate synthase beta subunit (Pseudomonas aeruginosa PAO1)
Anthranilate synthase. [EC: 4.1.3.27]

Predicted SEED Role

"Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85)" in subsystem Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. or Folate Biosynthesis or Tryptophan synthesis (EC 2.6.1.85)

MetaCyc Pathways

• superpathway of chorismate metabolism (51/59 steps found)
• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• L-tryptophan biosynthesis (6/6 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• 4-aminobenzoate biosynthesis I (2/2 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• 4-hydroxy-2(1H)-quinolone biosynthesis (2/5 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
• acridone alkaloid biosynthesis (1/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (1/4 steps found)
• superpathway of candicidin biosynthesis (4/11 steps found)
• superpathway of quinolone and alkylquinolone biosynthesis (2/10 steps found)
• chloramphenicol biosynthesis (1/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Folate biosynthesis
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.85, 4.1.3.27

Use Curated BLAST to search for 2.6.1.85 or 4.1.3.27

Protein Sequence (193 amino acids)

>CSW01_13255 aminodeoxychorismate/anthranilate synthase component II (Vibrio cholerae E7946 ATCC 55056)
MLLMIDNYDSFTYNLYQYFCELGAQVKVVRNDEIDLDGIRALAPTHLVISPGPCTPNEAG
ISLAAIEAFAGQLPILGVCLGHQAIAQAFGGQVVRARQVMHGKTSPIRHTGQGLFSGLNN
PLTVTRYHSLVVKNDTLPECFELTAWTELSDGSIDEIMGFQHKTLALEAVQFHPESIKTE
QGHQLLANFLNRT