Protein Info for CSW01_13215 in Vibrio cholerae E7946 ATCC 55056

Annotation: hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 329 PF12146: Hydrolase_4" amino acids 59 to 274 (216 residues), 49.6 bits, see alignment E=5e-17 PF00561: Abhydrolase_1" amino acids 61 to 301 (241 residues), 94.8 bits, see alignment E=1.1e-30 PF12697: Abhydrolase_6" amino acids 64 to 299 (236 residues), 31.5 bits, see alignment E=4.5e-11

Best Hits

Swiss-Prot: 53% identical to YHET_ECOLI: Putative esterase YheT (yheT) from Escherichia coli (strain K12)

KEGG orthology group: K07019, (no description) (inferred from 100% identity to vcj:VCD_001753)

Predicted SEED Role

"Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase" in subsystem cAMP signaling in bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (329 amino acids)

>CSW01_13215 hydrolase (Vibrio cholerae E7946 ATCC 55056)
MITFIAAKGLKNPHLQTLLPRLLRKQPLFSAEWQTLFTPDGDFLDLAWSEDWRTPHAQRK
PLFVLFHGLEGSFKSPYANGLMHAFARQGWLSVMMHFRGCSGKPNHLARAYHSGETGDAR
FVLEYLRKQLPERPIVAVGVSLGGNMLANYLAQYRDDPIVTAATLISAPLDLAACSQRIE
QGFSKVYRAYLLSSLKKNAIAKYPLLENALPLSPERIRAIAKLAEFDDVITAPLHGFHDA
ADYYQQCSGIKQLTQIRVPTQIIHAKDDPFMTEAVIPDFSLPANIDYRLFEHGGHVGFLS
GSWLKPYFWLEQALPEYYAPLRDDFLQPS