Protein Info for CSW01_12575 in Vibrio cholerae E7946 ATCC 55056

Annotation: transcriptional regulator LeuO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 PF00126: HTH_1" amino acids 27 to 85 (59 residues), 66.6 bits, see alignment E=1.6e-22 PF03466: LysR_substrate" amino acids 118 to 310 (193 residues), 80.2 bits, see alignment E=1.5e-26

Best Hits

Swiss-Prot: 54% identical to LEUO_ECOLI: HTH-type transcriptional regulator LeuO (leuO) from Escherichia coli (strain K12)

KEGG orthology group: K05798, LysR family transcriptional regulator, transcriptional activator for leuABCD operon (inferred from 100% identity to vch:VC2485)

Predicted SEED Role

"Probable transcriptional activator for leuABCD operon" in subsystem Leucine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (321 amino acids)

>CSW01_12575 transcriptional regulator LeuO (Vibrio cholerae E7946 ATCC 55056)
MQMLDKKDAMSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAV
ARLKVMFNDELFMRQGRGIQPTQRARQLFGPIRQALQLIRNELPSSVFTPETSTRLFKLA
ICSPCDLRFAPQIMASIDELAPSVQLHLDAEFDRQIAERMRYQEIDFVIDYARFDDQGFS
STEIFQDELVVVASKLHPHIQGSITAEQLSAEKHAKLSKVHGQRSFSELAYRDFDCQSYY
EGTSLSNVLYVVGQSELVTVAPRWMAENAANRDELQILAFPFAESKISGYLSWHESSEKD
KGHIWLRDQLMVICGEVVANR