Protein Info for CSW01_12340 in Vibrio cholerae E7946 ATCC 55056

Annotation: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 TIGR02198: bifunctional protein RfaE, domain I" amino acids 7 to 312 (306 residues), 418 bits, see alignment E=3.3e-129 PF00294: PfkB" amino acids 13 to 302 (290 residues), 156.6 bits, see alignment E=1.5e-49 TIGR02199: bifunctional protein RfaE, domain II" amino acids 332 to 472 (141 residues), 205 bits, see alignment E=6.7e-65 TIGR00125: cytidyltransferase-like domain" amino acids 342 to 408 (67 residues), 62.7 bits, see alignment E=4.1e-21 PF01467: CTP_transf_like" amino acids 344 to 469 (126 residues), 71.6 bits, see alignment E=1.1e-23

Best Hits

Swiss-Prot: 100% identical to HLDE_VIBCM: Bifunctional protein HldE (hldE) from Vibrio cholerae serotype O1 (strain M66-2)

KEGG orthology group: K03272, D-beta-D-heptose 7-phosphate kinase / D-beta-D-heptose 1-phosphate adenosyltransferase [EC: 2.7.1.- 2.7.7.-] (inferred from 100% identity to vco:VC0395_A2014)

MetaCyc: 70% identical to fused heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-4342 [EC: 2.7.7.70]; RXN0-4341 [EC: 2.7.7.70, 2.7.1.167]

Predicted SEED Role

"ADP-heptose synthase (EC 2.7.-.-) / D-glycero-beta-D-manno-heptose 7-phosphate kinase" in subsystem LOS core oligosaccharide biosynthesis (EC 2.7.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.-, 2.7.7.-

Use Curated BLAST to search for 2.7.-.- or 2.7.1.- or 2.7.1.167 or 2.7.7.- or 2.7.7.70

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (476 amino acids)

>CSW01_12340 bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase (Vibrio cholerae E7946 ATCC 55056)
MKPVLPDYSKAGVLIVGDVMLDRYWYGPTGRISPEAPVPVVKVEQSEERPGGAANVAMNI
ASLGGHAHIIGLTGQDEPANVLANKLTSLKVHCDFVALPDYPTITKLRVLSRGQQLIRLD
FEDKFENTDAQLILSRMESALPKVRAVILSDYAKGALEHVQQFIQKAKAAGVPVFIDPKG
SDFERYRGASLLTPNMSEFEAVVGKVKSEQELVEKGFALIEKFDLGALLVTRSEHGMTLL
RRGLEPFHLPTQAKEVYDVTGAGDTVISVLAASVAAGKALDEACALANAAAGVVVGKLGT
STVSTIELAEAVHGSKDTDYGVISEDALIEAVKKAQARGEKVVMTNGCFDILHAGHVSYL
NHAAELGDRLIVAVNTDESVKRLKGPGRPVNSTDRRMAVLAGLGAVDWVVPFSEDTPQRL
IAAVLPNLLVKGGDYKPEDIAGGKEVIAAGGEVKVLNFEEGCSTTEIIEAIKGGRG