Protein Info for CSW01_12305 in Vibrio cholerae E7946 ATCC 55056

Annotation: DNA topoisomerase IV subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 761 TIGR01062: DNA topoisomerase IV, A subunit" amino acids 10 to 744 (735 residues), 1244.1 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 34 to 470 (437 residues), 484.5 bits, see alignment E=3.1e-149 PF03989: DNA_gyraseA_C" amino acids 599 to 641 (43 residues), 22.4 bits, see alignment 6.5e-09 amino acids 647 to 681 (35 residues), 25 bits, see alignment (E = 1e-09)

Best Hits

Swiss-Prot: 70% identical to PARC_ECOLI: DNA topoisomerase 4 subunit A (parC) from Escherichia coli (strain K12)

KEGG orthology group: K02621, topoisomerase IV subunit A [EC: 5.99.1.-] (inferred from 82% identity to vsa:VSAL_I2669)

Predicted SEED Role

"Topoisomerase IV subunit A (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (761 amino acids)

>CSW01_12305 DNA topoisomerase IV subunit A (Vibrio cholerae E7946 ATCC 55056)
MSTEISFDGVEQLPLRKFTEDAYLNYSMYVIMDRALPYIGDGLKPVQRRIIYAMSELGLS
ASAKYKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDGQGNWGAPDDPKSF
AAMRYTEAKLSKFAEILLSELGQGTVDWQPNFDGTMQEPKMLPARLPHILLNGVTGIAVG
MATDIPPHNVREVADATIHLIDNPNAPLTDLMQYVKGPDFPTEAEIITPTVELEKIYRSG
RGSIKMRAVWCKEGSDIVITALPHQVSGAKLLEQIAAQMRAKKLPMVEDLRDESDHENPT
RIVIVPRSNRVDCDLLMNHLFASTDLEKSFRVNLNMIGLDNRPQVKGLVQILSEWISFRR
ETVRSRLQYRLDKVLARLHILQGLLIAYLNLDEVIEIIRNEDDPKAVLMARFDLTDIQAD
AILDTKLRHLAKLEEMKIRGEQDELEKERKKLEELLGSERRLNNLLKKEIKADADKFGDD
RRSPLVEREEAKALTERDLMPSEAITVVLSEKGWIRHAKGHDVDCQSLNYKAGDNYLTHA
CGKSNQQAVFLGSDGRSYSLESHTLPSARGQGEPITGRLNVAEGTTIRQVVMGEEDQLWL
VGSDAGYGFVCKGDDLLSKNRSGKALINLPENSEVMTPQVIADLDNDEILAITNQGRMLL
FPIKDLPQLSKGKGNKIINIPAAKAKTREEVLSNLMVLPQGASITLYAGKRKLGLKVSDL
DNFRGERGRRGALLPRGLQRVTAIEIETSAEPESTPHSEDE