Protein Info for CSW01_12190 in Vibrio cholerae E7946 ATCC 55056

Annotation: penicillin-binding protein 3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 580 transmembrane" amino acids 30 to 50 (21 residues), see Phobius details PF03717: PBP_dimer" amino acids 74 to 227 (154 residues), 67 bits, see alignment E=3e-22 PF00905: Transpeptidase" amino acids 267 to 562 (296 residues), 270.4 bits, see alignment E=2e-84

Best Hits

Swiss-Prot: 51% identical to FTSI_ECO57: Peptidoglycan D,D-transpeptidase FtsI (ftsI) from Escherichia coli O157:H7

KEGG orthology group: K03587, cell division protein FtsI (penicillin-binding protein 3) [EC: 2.4.1.129] (inferred from 100% identity to vch:VC2407)

MetaCyc: 51% identical to peptidoglycan DD-transpeptidase FtsI (Escherichia coli K-12 substr. MG1655)
Serine-type D-Ala-D-Ala carboxypeptidase. [EC: 3.4.16.4]

Predicted SEED Role

"Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)" in subsystem Bacterial Cell Division or Bacterial Cytoskeleton or Flagellum in Campylobacter or Peptidoglycan Biosynthesis (EC 2.4.1.129)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.16.4

Use Curated BLAST to search for 2.4.1.129 or 3.4.16.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (580 amino acids)

>CSW01_12190 penicillin-binding protein 3 (Vibrio cholerae E7946 ATCC 55056)
MKKKAPAKASRVSPRVTTAEPAPIFIRWRFYLLLFFVLTAFCALVARVAYIQIIEPDNLI
KEGDLRSIRAKTLQSARGIISDRNGEALAVSVPVEAVWADPVAIYKEGGLVEKDRWYALA
DVLGLDRQEMMKKIEDNRSRRFIYLQRQVSPAMAKYIRELKLAGIGLKEESRRYYPAGEV
SAHLVGVTGIDGHGLEGVERSFDSTLTGESGKSVTRKDKFGRVVENIALEEREEGKPIQL
TIDQRLQAIAFRAVKQAMADYRATSASAVMLDVKTGAVLAMVNAPSYNPNNRNDWQSFKM
RNRVITDAFEPGSTVKPFVVLAALANGIADKNTIIDTGDGTMQIGGSRVRDTSKVGKADL
TLILKKSSNIGVAKLALEMPLEALLGMYSSVGLGEMSGLDLVGETIGIFPNRRRWSQFEI
ATLSFGYGLAITPIQLAHAYATLGNHGKYQPIHIIKGDQHGETRQVVDPKYVNQVLEMLE
TVTQPGGTATRAAVPGYRIAAKSGTSRKAIAGGYSDEYFAYTAGVAPVSDPRISLVVMVN
EPQGDSYYGGAVAGPVFSEIMKGALQILNIAPDENRFQNK