Protein Info for CSW01_12180 in Vibrio cholerae E7946 ATCC 55056

Annotation: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 24 to 73 (50 residues), 29.9 bits, 9.1e-11
• TIGR01143: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
  • amino acids 29 to 448 (420 residues), 495 bits, E=9.4e-153
• PF08245: Mur_ligase_M
  • amino acids 105 to 293 (189 residues), 158.6 bits, E=3.1e-50
• PF02875: Mur_ligase_C
  • amino acids 314 to 393 (80 residues), 34.3 bits, E=3.6e-12

Best Hits

Swiss-Prot: 53% identical to MURF_ECOLI: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (murF) from Escherichia coli (strain K12)

KEGG orthology group: K01929, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase [EC: 6.3.2.10] (inferred from 100% identity to vcj:VCD_001948)

MetaCyc: 53% identical to D-alanyl-D-alanine-adding enzyme (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase. [EC: 6.3.2.10]

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.10

Protein Sequence (453 amino acids)

>CSW01_12180 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase (Vibrio cholerae E7946 ATCC 55056)
MIRVMLSQLSSVLNARLIGEDRAVSAVSTDSRAIPAEALFVALVGERFDAHDFASQAVTG
GAVALLVERELDAECSQLVVADTKQALGQLGSWVHAQCQTPTVAITGSCGKTTVKEMVAS
ILSQKGQVLYTQGNFNNDIGVPLTLLRSTTEDDYAVIELGANHVGEIAYTTALVKPDVAL
VNNVAAAHLEGFGSMDGVKRAKGEIYQGLQAGAVAIVNLDSNGGDYWKAVLADKTVKTFS
ATDHNADFYPRDVRLNALGIASFTLVTPVGEVGVELGIMGQHNVANALAAAALSLEMGAS
LAEIGQGLAHLSKVKGRVDMAQLRDDITLIDDSYNASVPAMKAAVELLGSFSAVRWLILG
NMAELGDESLALHQQVGEHAAPFGFEFVLTYGKDAKVISDLCQGHHFATHQDMMTFIEQH
IEQQQGRTQVLLVKGANSAGMSKIAAALKEKFA