Protein Info for CSW01_12100 in Vibrio cholerae E7946 ATCC 55056

Annotation: carbamoyl-phosphate synthase large chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1050 (1049 residues), 1593.1 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 128 to 334 (207 residues), 243.9 bits, E=5e-76
  • amino acids 673 to 875 (203 residues), 133.8 bits, E=2.5e-42
• PF02787: CPSase_L_D3
  • amino acids 427 to 505 (79 residues), 93.4 bits, 2.7e-30
• PF02655: ATP-grasp_3
  • amino acids 672 to 845 (174 residues), 36.8 bits, E=1.7e-12
• PF02222: ATP-grasp
  • amino acids 682 to 845 (164 residues), 34.8 bits, E=5.2e-12
• PF07478: Dala_Dala_lig_C
  • amino acids 689 to 843 (155 residues), 30.4 bits, E=1.1e-10
• PF02142: MGS
  • amino acids 956 to 1041 (86 residues), 71.8 bits, E=1.7e-23

Best Hits

Swiss-Prot: 70% identical to CARB_BUCAI: Carbamoyl-phosphate synthase large chain (carB) from Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 74% identity to abo:ABO_0318)

MetaCyc: 86% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• UMP biosynthesis I (6/6 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• L-arginine degradation V (arginine deiminase pathway) (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of arginine and polyamine biosynthesis (13/17 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (1/4 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (1076 amino acids)

>CSW01_12100 carbamoyl-phosphate synthase large chain (Vibrio cholerae E7946 ATCC 55056)
MPKRTDIQSILILGAGPIVIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPEM
ADATYIEPIHWEVVRKIIEKERPDAILPTMGGQTALNCALALEKHGVLAEFGVEMIGATA
DAIDKAEDRSRFDKAMKSIGLECPRADTAKSMEEAYKVLDMVGFPCIIRPSFTMGGSGGG
IAYNREEFEEICTRGLDLSPTNELLIDESLIGWKEYEMEVVRDKNDNCIIVCAIENFDPM
GIHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIGVETGGSNVQFGINPKDGRMVIIEM
NPRVSRSSALASKATGFPIAKVAAKLAVGFTLDELMNDITGGATPASFEPTIDYVVTKIP
RFNFEKFAGANDRLTTQMKSVGEVMAIGRNQQESLQKALRGLEVGAAGLDEKVDLDAPDA
LTKIRYELKEAGAERIWYIADAFRAGMSVDGVFNLTNIDRWFLVQIEELVKLEAEVKAGG
FAGLNQDVLRKMKRKGFSDARLSKLLGVSENEIRRLRDQYNIHPVYKRVDTCAAEFKSDT
AYMYSTYDEECEANPTDKDKIMVLGGGPNRIGQGIEFDYCCVHAALALREDGYETIMVNC
NPETVSTDYDTSDRLYFEPVTLEDVLAIVRVEKPKGVIVQYGGQTPLKLARALEAAGVPV
IGTSPDAIDRAEDRERFQQAVQRLGLKQPDNATVTAIEQAIEKSREIGFPLVVRPSYVLG
GRAMEIVYDEQDLRRYFNEAVSVSNESPVLLDRFLDDATEVDVDAICDGERVVIGGIMEH
IEQAGVHSGDSACSLPAYTLSQEIQDKMREQVEKLAFELGVRGLMNIQFAVKDNEVYLIE
VNPRAARTVPFVSKATGAPLAKIAARVMVGQTLEQQGFTKEIIPPYYSVKEVVLPFNKFP
GVDPLLGPEMRSTGEVMGVGATFAEAYAKAELGCGSVYPEGGRALLSVREGDKQRVVDLA
SKLVKLGYQLDATHGTAVILGEAGINPRLVNKVHEGRPHILDRIKNHEYTYIVNTASGRQ
AIEDSKVLRRGALAHKVNYTTTLNAAFATCMSHTADAKASVTSVQELHARVKANQA