Protein Info for CSW01_12030 in Vibrio cholerae E7946 ATCC 55056

Annotation: glutamate synthase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00310: GATase_2
  • amino acids 13 to 426 (414 residues), 598.6 bits, E=1.2e-183
• PF04898: Glu_syn_central
  • amino acids 458 to 738 (281 residues), 353 bits, E=2.4e-109
• PF01645: Glu_synthase
  • amino acids 794 to 1157 (364 residues), 499.7 bits, E=1.2e-153
• PF01493: GXGXG
  • amino acids 1236 to 1420 (185 residues), 249.2 bits, E=4.5e-78

Best Hits

Swiss-Prot: 73% identical to GLTB_ECOLI: Glutamate synthase [NADPH] large chain (gltB) from Escherichia coli (strain K12)

MetaCyc: 73% identical to glutamate synthase subunit GltB (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (1/4 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13

Use Curated BLAST to search for 1.4.1.13

Protein Sequence (1487 amino acids)

>CSW01_12030 glutamate synthase large subunit (Vibrio cholerae E7946 ATCC 55056)
MALYDPSLEKDNCGFGLIAHMEGQASHKLVRTAISALDRMTHRGGIAADGKTGDGCGLLL
QKPDSYLRLIAEEQHWKLSKQYAIGMLFLSRDPHKAQLAQQIVNQELAKETLSVAGWRKV
PTNPKVLGPIALDSLPDIQQVFISAPAGWRERDVERRLYIARRRIEKQITDDRDFYICSL
STQVLVYKGLCMPADLPRFYLDLADLRMESAICLFHQRFSTNTQPRWPLAQPFRYLAHNG
EINTIEGNRQWARARAYKFASPLLPDLQSAAPFVNETGSDSSSLDNMLDLFLAGGMDIFR
AMRMLVPPAWQNHPDMDPDLRAFYDFNSKHMEPWDGPAGIVLSDGRYAACNLDRNGLRPA
RYVITKDKLITLASEVGIWDYAPDEVSEKGRVGPGELLVIDTRKGKIWQSSEIDNDLKSR
HPYREWMENNVHKLTPFSQLPDDKVGERSFDADLLKTYQKQFAMSNEEIDQILRVLGDMA
QEAVGSMGDDTPMAVLSSKERLISDYFRQKFAQVTNPPIDPLREKHVMSLATSIGQEMNV
FCETDGHAHRVTFDSPILLYSDMQQLLTLSDQHYRNTILDINFDPQEKNLKQAVLDLCDK
AEQVVREGTVLVVLSDRALTADRLPIPAAMAVGAVQARLVEANLRCDANIIIETGAARDP
HHFAVLIGFGATAVYPYLAYETLGKMIDDGALQKSYREVMQNYQYGINKGLYKIMSKMGI
STVASYRCSQLFEAVGLHRDVVDLCFKGVTTRIQGANFDDFEQDLFNLSRKAWAKRKPLE
HGGLLKYVHGGEYHAYNPDVVGTLQKAVKSGEIMDYREFAQQVNQRPVAMLRDLLRLKTS
DKPLPLEHIEPASDLYKRFDSAAMSIGALSPEAHEALATAMNRLGGYSNSGEGGEDPRRF
GTERNSRIKQVASGRFGVTPHYLTNADVLQIKVAQGAKPGEGGQLPGHKVTAEIAKLRYS
VPGVTLISPPPHHDIYSIEDLAQLIFDLKQINPKALVSVKLVSEPGVGTIATGVAKAYAD
LITISGYDGGTAASPLTSVKYAGSPWELGLAETQQALVANGLRHKIRLQVDGGLKTGLDV
IKGAILGAESFGFGTAPMVAMGCKFLRICHLNNCATGVATQDETLRKDYFKGLPEMVMNY
FKGLAEEVRGYLAELGVEKLTDLIGRTDLLEVVEGMTAKQSKLDLSDLLEAPVSPQNLPL
YCTEPNTPFDKGALNQKIVEDALAAVENQQSLELYYNVINTDRSIGARLSGEIAKRYGNL
GVATSPIKVVLNGTAGQSFGVWNAGGLELYLTGDANDYVGKGMAGGKIVIRPHQGTAFVC
NEATIIGNTCLYGATGGKLFAAGKAGERFAVRNSGTIAVIEGAGDNACEYMTGGIVAILG
ATGVNFGAGMTGGFAYVLDENGDFQGRVNDESVEAVALTDLYIHQEHLRGLIAEHLEETG
SAHAERILANFDEWIPKFYLIKPQAADLRTLLGHQSRSAAELRVQAQ