Protein Info for CSW01_11975 in Vibrio cholerae E7946 ATCC 55056

Annotation: bifunctional aspartate kinase/homoserine dehydrogenase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00696: AA_kinase
  • amino acids 2 to 284 (283 residues), 172.9 bits, E=2.3e-54
• TIGR00657: aspartate kinase
  • amino acids 3 to 460 (458 residues), 432 bits, E=1.5e-133
• PF13840: ACT_7
  • amino acids 392 to 456 (65 residues), 29.6 bits, E=1.3e-10
• PF03447: NAD_binding_3
  • amino acids 472 to 606 (135 residues), 82.6 bits, E=8.9e-27
• PF00742: Homoserine_dh
  • amino acids 614 to 810 (197 residues), 169.3 bits, E=1.8e-53

Best Hits

Swiss-Prot: 62% identical to AK1H_SERMA: Bifunctional aspartokinase/homoserine dehydrogenase 1 (thrA) from Serratia marcescens

KEGG orthology group: K12524, bifunctional aspartokinase / homoserine dehydrogenase 1 [EC: 1.1.1.3 2.7.2.4] (inferred from 100% identity to vcj:VCD_001985)

MetaCyc: 64% identical to fused aspartate kinase/homoserine dehydrogenase 1 (Escherichia coli K-12 substr. MG1655)
Aspartate kinase. [EC: 2.7.2.4]; Homoserine dehydrogenase. [EC: 2.7.2.4, 1.1.1.3]

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis or Methionine Biosynthesis (EC 1.1.1.3, EC 2.7.2.4)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• norspermidine biosynthesis (6/6 steps found)
• superpathway of L-threonine biosynthesis (6/6 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• ectoine biosynthesis (5/5 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• superpathway of polyamine biosynthesis III (7/8 steps found)
• L-lysine biosynthesis III (6/7 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• L-homoserine biosynthesis (3/3 steps found)
• dipicolinate biosynthesis (3/4 steps found)
• spermidine biosynthesis II (3/4 steps found)
• L-lysine biosynthesis II (6/9 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• L-methionine biosynthesis IV (2/4 steps found)
• 3-dehydroquinate biosynthesis II (archaea) (3/7 steps found)
• cremeomycin biosynthesis (2/7 steps found)
• grixazone biosynthesis (2/8 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones
• Glycine, serine and threonine metabolism
• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.3, 2.7.2.4

Use Curated BLAST to search for 1.1.1.3 or 2.7.2.4

Protein Sequence (819 amino acids)

>CSW01_11975 bifunctional aspartate kinase/homoserine dehydrogenase I (Vibrio cholerae E7946 ATCC 55056)
MRVLKFGGSSLADPERFLRAADIIANNAQQEEVAVVLSAPGKTTNKLVAVIESALRQGEV
EAQIVELESSFYALLDGIKAQLPNLDDSAYQQQVHSSMTQLRQFVHGITLLGMCPDNVNA
RIISKGERVSIQLMKAVMEAKGLPANLVDPVKYLLAKGDHLEAMVDVEISTQRFRQAPLP
QQHVNIMPGFTAGNAQGELVCLGRNGSDYSAAVLAACLRADCCEIWTDVDGVYNCDPRLV
DDARLLKSLSYQEAMELSYFGASVLHPKTIAPIAQFQIPCLIKNSFNPQGAGTLIGQDTG
EDKLAIKGITTLSNLTMVNVSGPGMKGMVGMASRVFGAMSAADVSIVLITQSSSEYSISF
CIEAQHKALAQQALADAFELELKDGLLEPVEFVDNVAIITLVGDGMRTSRGVASQFFSSL
AEVHVNVIAIAQGSSERAISAVIPDDKISEAIKACHENLFNSKHFLDVFVVGVGGVGGEL
VDQIQRQQAKLAEKGIMMRVCGLANSKGMLLDSQGLPLEQWRDRMINADQAFSLENLVAL
VQRNHIINPVLVDCTSSDEIANQYADFLAAGFHVVTPNKKANTASMSYYHQLRNVARHSR
RKLMYETTVGAGLPVIENLQNLIAAGDELEKFSGILSGSLSFIFGKLDEGMTLSQATQLA
KEKGFTEPDPRDDLSGMDVARKLLILAREAGYELELSDVDVEQALPAGFDASGSVEEFMA
RLAQADAAFAERVAQAKAEGKVLRYVAQIVDGQCQVRIVAVDENDPMFKVKEGENALAFY
SRYYQPIPLVLRGYGAGSEVTAAGVFSDVMRTLGWKLGV