Protein Info for CSW01_11845 in Vibrio cholerae E7946 ATCC 55056

Annotation: beta-galactosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1024 PF02837: Glyco_hydro_2_N" amino acids 48 to 215 (168 residues), 193.3 bits, see alignment E=7.8e-61 PF00703: Glyco_hydro_2" amino acids 218 to 331 (114 residues), 51.5 bits, see alignment E=4.1e-17 PF02836: Glyco_hydro_2_C" amino acids 333 to 626 (294 residues), 379.3 bits, see alignment E=3.1e-117 PF16353: LacZ_4" amino acids 634 to 719 (86 residues), 54.6 bits, see alignment E=2.8e-18 PF02929: Bgal_small_N" amino acids 748 to 1016 (269 residues), 235.5 bits, see alignment E=2e-73

Best Hits

Swiss-Prot: 100% identical to BGAL_VIBC3: Beta-galactosidase (lacZ) from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)

KEGG orthology group: K01190, beta-galactosidase [EC: 3.2.1.23] (inferred from 100% identity to vcm:VCM66_2261)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1024 amino acids)

>CSW01_11845 beta-galactosidase (Vibrio cholerae E7946 ATCC 55056)
MRNFSDILLSQDWQNPHIVKWHCRTPHVPLHSYRTEQEARLDVGGNRQSLNGQWRFALFE
KPEAVEPAVIDPDFDDSAWAHIPVPSNWQMQGFDKPIYTNIQYPFADRPPYVPQDNPTGC
YRHRFTLEKQALTESIRIVFDGVNSAFHLWCNGHWVGYSQDSRLPAEFELTPYLQEGENL
LVAMVLRWSDGSYLEDQDMWWLSGIFRDVYLYRKPILAIEDFFIRTELDALYQHAELRVE
TRLSQVTRHHQVQVALFDAQGECVARSQALHTGQRVVDEKGAWHDKTEHSLAICSPTLWS
DEAPYLYRCVICLLDEDGAPIEFESAAVGFRKVEITQGLLKLNGQPLLIRGVNRHEHHPE
LGHVMDEASMRRDIELMKQHNFNAVRTAHYPNHPRWYELCDEYGLYVVDEANLETHGQFP
MSRLSNDPQWVNAYLQRMIGMVERDKNHPCVIIWSLGNESGIGTNHHAMYQWTKQRDPSR
PVQYEGGGANTAATDIVCPMYARVDQHQPHPAVPKYALKNWISLPQENRPLILCEYAHAM
GNSLGAFYKYWQAFREFPRLQGGFIWDWVDQGISKWDSEGRHYWGYGGDFGDTINDRQFC
INGLLFPDRTPHPALHEVKKVQQPYQFSLSYPKLTIHNERLFAALPLELVVSVLCDGQEI
KQERLPLDIAPRGTITLDLASLPMLPEHEYHLNAVLLCREDQPWSNAGHCIASEQWCLQP
RRSMLPKITHAPLPQWQQDGDKVRIEAANQQWQFNRQTGLLEQWWQNGQPVLSEPLRDNF
YRAVLDNDIGTSEAQHLDPNSWIARWHAAGLDKLRVECDDLRVTTLNESVEVVIDVAHYH
QQALALRTRWRYQIFGDARVELNVEVMLCSDLPPLPRVGLTLALPVAENPVSWFGRGPHE
NYPDRLQSAHVGRYTATVDELHTPYIFPSENGLRCDTRQLQVGALVVEGHFHFSLSRYSQ
TMLDKAKHSNELVAGDKWYLNLDAQHMGVGGDDSWSQSVHPEFLLTQPHYQYQLTLRVKA
SSPQ