Protein Info for CSW01_11550 in Vibrio cholerae E7946 ATCC 55056

Annotation: bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00506: 3,4-dihydroxy-2-butanone-4-phosphate synthase
  • amino acids 7 to 199 (193 residues), 251.7 bits, E=2.3e-79
• PF00926: DHBP_synthase
  • amino acids 7 to 198 (192 residues), 294 bits, E=4.1e-92
• PF00925: GTP_cyclohydro2
  • amino acids 207 to 367 (161 residues), 159.9 bits, E=4.3e-51

Best Hits

Swiss-Prot: 66% identical to RIBB_PHOPO: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) from Photobacterium phosphoreum

KEGG orthology group: K14652, 3,4-dihydroxy 2-butanone 4-phosphate synthase / GTP cyclohydrolase II [EC: 3.5.4.25 4.1.99.12] (inferred from 100% identity to vcj:VCD_002073)

Predicted SEED Role

"3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25)" in subsystem Molybdenum cofactor biosynthesis or Riboflavin, FMN and FAD metabolism (EC 3.5.4.25, EC 4.1.99.12)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia) (3/5 steps found)
• toxoflavin biosynthesis (3/7 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.25, 4.1.99.12

Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12

Protein Sequence (369 amino acids)

>CSW01_11550 bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II (Vibrio cholerae E7946 ATCC 55056)
MPISTPQEIIEDIRQGKMVILMDDEDRENEGDLIMAAEHITPAAINFMATHGRGLICLTL
TKERCRRLGLNPMVQDNNAQYTTNFTVSIEAAEGVTTGISAADRARTVQAAVAKEAKAAD
LVQPGHIFPLAAQDGGVLTRAGHTEAGCDLARLAGLEPASVIVEILNDDGTMARRPDLEV
FAEKHGLKLGTIADLIEYRNHTETTIERVAQCKLPTEYGEFELVTYRDIIDKQIHFALRK
GDITSAPTLVRVHLQDTFTDLLHSNRAAERSWPLATAMQRIGQEGGVLVILGHEESSELL
LHRVKMFELQDKGEAPAMAKKQGTSRRVGVGSQILADLGVKEMRLLSSPNKKYHALGGFG
LNVVEYVCE