Protein Info for CSW01_11380 in Vibrio cholerae E7946 ATCC 55056

Annotation: DNA polymerase III subunit epsilon

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 247 TIGR00573: exonuclease, DNA polymerase III, epsilon subunit family" amino acids 10 to 230 (221 residues), 274.5 bits, see alignment E=5.6e-86 TIGR01406: DNA polymerase III, epsilon subunit" amino acids 11 to 246 (236 residues), 354 bits, see alignment E=3.9e-110 PF00929: RNase_T" amino acids 13 to 180 (168 residues), 143.9 bits, see alignment E=3.4e-46

Best Hits

Swiss-Prot: 62% identical to DPO3E_SALTY: DNA polymerase III subunit epsilon (dnaQ) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02342, DNA polymerase III subunit epsilon [EC: 2.7.7.7] (inferred from 100% identity to vch:VC2233)

MetaCyc: 60% identical to DNA polymerase III subunit epsilon (Escherichia coli K-12 substr. MG1655)
3.1.11.-

Predicted SEED Role

"DNA polymerase III epsilon subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (247 amino acids)

>CSW01_11380 DNA polymerase III subunit epsilon (Vibrio cholerae E7946 ATCC 55056)
MNTSNNAEYQRIVVLDTETTGMNREGGPHYEGHRIIEIGAVEIINRKLTGRHFHVYLKPD
RDIQLEAIEVHGITDEFLKDKPEYKDVHEEFVDFIKGAELVAHNAPFDVGFMDYEFAKLG
GAIGKTSDFCKVTDTLAMAKRIFPGKRNNLDILCERYGIDNSHRTLHGALLDAEILADVY
LLMTGGQTSLQFSSVTQNSGELSAESLKRARSERKALKVLAASADELQAHQDRLDIVAKS
GTCLWRS