Protein Info for CSW01_11335 in Vibrio cholerae E7946 ATCC 55056

Annotation: 23S rRNA pseudouridine(2604) synthase RluF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 340 PF01479: S4" amino acids 20 to 64 (45 residues), 36.7 bits, see alignment 2.8e-13 PF00849: PseudoU_synth_2" amino acids 79 to 205 (127 residues), 65.6 bits, see alignment E=6.3e-22 TIGR00093: pseudouridine synthase" amino acids 83 to 235 (153 residues), 158.8 bits, see alignment E=4.8e-51

Best Hits

KEGG orthology group: K06182, ribosomal large subunit pseudouridine synthase F [EC: 5.4.99.12] (inferred from 100% identity to vcm:VCM66_2146)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase F (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (340 amino acids)

>CSW01_11335 23S rRNA pseudouridine(2604) synthase RluF (Vibrio cholerae E7946 ATCC 55056)
MCTIRCETLGDCMSQPTEKRLNKFISETGYCSRREADKLIEQGRVTLNGLQPEVGSKVTE
QDEVCIDGKPLRSKERPIYIALNKPTGITCTTERDVPGNIVDFIGHHKRIFPIGRLDKPS
DGLIFLTNDGDIVNKILRAGNNHEKEYVVRVDKPITPEFIRQMSSGVNILDTVTLPCKVT
QETQYSFRIVLTQGLNRQIRRMCEALGYEVFKLRRVRIMNITLDGIPNGKWRYLSEAEVS
EILAMCEDSSGTEEASRVAQSGDTIKRATDAKLFDSREANKESKARRNPKTYHGKNAQEF
RHAPNSKKAQRRPSSPSSNTASGSKPTPRTGGTLSLRKSK