Protein Info for CSW01_10840 in Vibrio cholerae E7946 ATCC 55056

Annotation: protein-(glutamine-N5) methyltransferase, release factor-specific

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 286 TIGR00536: methyltransferase, HemK family" amino acids 4 to 282 (279 residues), 247.1 bits, see alignment E=1.9e-77 PF17827: PrmC_N" amino acids 8 to 77 (70 residues), 76.3 bits, see alignment E=7.6e-25 TIGR03534: protein-(glutamine-N5) methyltransferase, release factor-specific" amino acids 27 to 276 (250 residues), 289.3 bits, see alignment E=2.5e-90 PF06325: PrmA" amino acids 106 to 188 (83 residues), 27.9 bits, see alignment E=6e-10 PF05175: MTS" amino acids 107 to 198 (92 residues), 54 bits, see alignment E=5.8e-18 PF13847: Methyltransf_31" amino acids 116 to 191 (76 residues), 48.4 bits, see alignment E=2.8e-16 PF08241: Methyltransf_11" amino acids 118 to 187 (70 residues), 30.4 bits, see alignment E=1.7e-10 PF13649: Methyltransf_25" amino acids 118 to 188 (71 residues), 43.8 bits, see alignment E=1.2e-14

Best Hits

Swiss-Prot: 100% identical to PRMC_VIBCH: Release factor glutamine methyltransferase (prmC) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K02493, methyltransferase [EC: 2.1.1.-] (inferred from 100% identity to vcj:VCD_002200)

MetaCyc: 54% identical to protein-(glutamine-N5) methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-14992 [EC: 2.1.1.297]

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.297

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (286 amino acids)

>CSW01_10840 protein-(glutamine-N5) methyltransferase, release factor-specific (Vibrio cholerae E7946 ATCC 55056)
MSVTIEAALKAATEQLQQSGSDSPALDAAVLLCHVLAKPRSYLLTWPDKILEKPTLASLE
LLLARRRAGEPMAYILGEREFWSLPLKVSPSTLIPRPDTERLVELALDKAALIDGELLDL
GTGTGAIALALASELPTRQVTGIDLRPEAAELARENATRLAIHNAQFFQGSWFSPLADGT
KFALIVSNPPYIEENDPHLSLGDVRFEPKSALVAAENGLADIRHISTHAPHFLLDGGWLL
FEHGYDQGVAVRTILRDLGYQNIITEQDYAGHDRVTLGQYKTEREA