Protein Info for CSW01_10535 in Vibrio cholerae E7946 ATCC 55056

Annotation: bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 674 PF05430: Methyltransf_30" amino acids 119 to 245 (127 residues), 158.1 bits, see alignment E=9.3e-51 TIGR03197: tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain" amino acids 268 to 667 (400 residues), 523.7 bits, see alignment E=1.7e-161 PF01266: DAO" amino acids 269 to 637 (369 residues), 175.8 bits, see alignment E=1.9e-55

Best Hits

Swiss-Prot: 100% identical to MNMC_VIBCH: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: None (inferred from 100% identity to vcm:VCM66_2033)

Predicted SEED Role

"tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) / FAD-dependent cmnm(5)s(2)U34 oxidoreductase" (EC 2.1.1.61)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.61

Use Curated BLAST to search for 2.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (674 amino acids)

>CSW01_10535 bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC (Vibrio cholerae E7946 ATCC 55056)
MFIMSSISHAQLGWNDAGTPVSDQFDDVYFSNVNGLAETRYVFLEQNHLPQRWHNDDQRR
FVIAETGFGTGLNFLAVWQAFVAFREANPDAKLKELHFISFEKYPLSKSDLIQAHQAWPE
LAQFAQKLHKHYPLAIPECQRIVLNDGLVTLDLWFGDIKDCLPKVATQEQGLVDAWFLDG
FAPSKNPEMWNQNLFAGMAKLAKHGCTCATFTSAGFVRRGLIDAGFAMKKVKGFGTKREM
IAGSLSEKVPYTNIAPEFRFEATHGLQEVAIIGGGIASATLATTLARRGVAVTLYCADEK
PAQGASGNRQGAVYPLLSGDHNAVSRVFAPAFLFARQWIEQAAEQINFDHDWCGVTQLMW
DEKATDKLKSMLEGNFPTQLVHGLSAEQTNQQVGVPVDKASVHYPLGGWLSPAELTQGLI
HLLEQQGKLTAHYQTPIDALTWQPETQLWQLRSGDTLMSHQCVVIASGHQFDSLSQTAEL
PLGKVKGQVSHIPTTETLSKINSVLCYDGYMTPVSQQNGYHCIGASYDRQHLDATFDPQA
QHENAQKLIHCLPEQTWPLEVDVSGNQSRQGVRCVSRDHLPFVGNVGEFSKITEQYRDLA
QQHQAEPIALYPQLYALVGLGSRGLSSAPLMAELLASQMCGDPMPLGVDLLEQLHPSRMW
VRKLRKGKALTQKV