Protein Info for CSW01_10525 in Vibrio cholerae E7946 ATCC 55056

Annotation: DUF3410 domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 387 PF00389: 2-Hacid_dh" amino acids 10 to 293 (284 residues), 82.6 bits, see alignment E=3.2e-27 PF02826: 2-Hacid_dh_C" amino acids 116 to 263 (148 residues), 123.9 bits, see alignment E=7.6e-40 PF11890: DUF3410" amino acids 296 to 376 (81 residues), 93.7 bits, see alignment E=7.8e-31

Best Hits

Swiss-Prot: 100% identical to PDXB_VIBCH: Erythronate-4-phosphate dehydrogenase (pdxB) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K03473, erythronate-4-phosphate dehydrogenase [EC: 1.1.1.290] (inferred from 100% identity to vch:VC2108)

MetaCyc: 51% identical to erythronate-4-phosphate dehydrogenase (Escherichia coli K-12 substr. MG1655)
4-phosphoerythronate dehydrogenase. [EC: 1.1.1.290]

Predicted SEED Role

"Erythronate-4-phosphate dehydrogenase (EC 1.1.1.290)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 1.1.1.290)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.290

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (387 amino acids)

>CSW01_10525 DUF3410 domain-containing protein (Vibrio cholerae E7946 ATCC 55056)
MPQIPTMKILIDENMPYAQALFSQLGEVILKPGRTLTADDLIDVDALMIRSVTKVNDALL
AKANRLKFVGTATAGMDHVDQALLRERGIFFTAAPGCNKVGVAEYVFSVLMVLAQQQGFS
VFDKTVGIIGAGQVGSYLAKCLSGIGMKVLLNDPPKQAQGDEREFTELETLLKQADVITL
HTPITRGGEWPTHHLIDAAILEQLRSDQILINAARGPVVDNAALKARLQQGDGFTAVLDV
FEFEPQVDMELLPLLAFATPHIAGYGLEGKARGTTMIFNSYCEFLGSAHCANPASLLPKA
PVPKVYLERAWDEETLRTLTQIIYDVRKDDAQFRREIHQPGAFDLMRKHYWDRREYSAVT
LAGGADCHLAPLAKLGFQVEVCDEPTI