Protein Info for CSW01_10370 in Vibrio cholerae E7946 ATCC 55056

Annotation: arginine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 599 TIGR00456: arginine--tRNA ligase" amino acids 25 to 599 (575 residues), 651.3 bits, see alignment E=5.9e-200 PF03485: Arg_tRNA_synt_N" amino acids 29 to 109 (81 residues), 70.4 bits, see alignment E=2.4e-23 PF00750: tRNA-synt_1d" amino acids 120 to 469 (350 residues), 536.2 bits, see alignment E=4.8e-165 PF05746: DALR_1" amino acids 483 to 599 (117 residues), 128 bits, see alignment E=2.9e-41

Best Hits

Swiss-Prot: 100% identical to SYR_VIBCH: Arginine--tRNA ligase (argS) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01887, arginyl-tRNA synthetase [EC: 6.1.1.19] (inferred from 100% identity to vco:VC0395_A1661)

MetaCyc: 72% identical to arginine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Arginine--tRNA ligase. [EC: 6.1.1.19]

Predicted SEED Role

"Arginyl-tRNA synthetase (EC 6.1.1.19)" (EC 6.1.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (599 amino acids)

>CSW01_10370 arginine--tRNA ligase (Vibrio cholerae E7946 ATCC 55056)
MICLTSKMMAFLPYFLELKGKRVNIQALINDRVSQAIEAAGAPAGTPALVRQSAKAQFGD
YQANGIMGAAKQLGTNPREFAQKVLDVLNLEGIASKTEIAGPGFINIFLSEEFLAAQAEA
ALADARLGVAQEAPKTIVADYSAPNVAKEMHVGHLRSTIIGDAVVRTLEFLGHKVIRANH
IGDWGTQFGMLIANLERVQKASGEVSMELSDLEAFYRESKKLYDEDEQFAETARNYVVKL
QGGDPFCLEMWKKLVDVTMIQNQRNYDRLNVSLTRENVMGESMYNDMLPQIVSDLKAKGL
AVEDDGAQVVFLEEFKNKDGEPMGVIIQKRDGGFLYTTTDIACAKYRYETLGADRVLYFI
DSRQHQHLMQAWTIVRKAGYIPENVSLEHHAFGMMLGKDGRPFKTRAGGTVRLADLLDEA
QERAKALIESKNPELSAEEKANIANTVAMAAVKYADLSKHRTTDYVFDWDNMLAFEGNTA
PYMQYAYTRVASVFAKAGVDMNELTGHIQITEEKEKALIAKLLQFEEAVQSVAREGQPHI
MCSYLFELAGIFSSFYEACPILVAEQESIKQSRLKLAALTAKTIKQGLALLGIDTLERM