Protein Info for CSW01_09945 in Vibrio cholerae E7946 ATCC 55056

Annotation: NADPH-dependent 2,4-dienoyl-CoA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 666 PF00724: Oxidored_FMN" amino acids 4 to 327 (324 residues), 308 bits, see alignment E=2.9e-95 PF07992: Pyr_redox_2" amino acids 374 to 625 (252 residues), 92.6 bits, see alignment E=9.7e-30 PF00070: Pyr_redox" amino acids 374 to 411 (38 residues), 25.2 bits, see alignment 5.8e-09 PF13450: NAD_binding_8" amino acids 377 to 413 (37 residues), 39.3 bits, see alignment 2e-13

Best Hits

Swiss-Prot: 56% identical to FADH_ECOLI: 2,4-dienoyl-CoA reductase (fadH) from Escherichia coli (strain K12)

KEGG orthology group: K00219, 2,4-dienoyl-CoA reductase (NADPH2) [EC: 1.3.1.34] (inferred from 100% identity to vch:VC1993)

MetaCyc: 56% identical to 2,4-dienoyl-CoA reductase (Escherichia coli K-12 substr. MG1655)
2,4-dienoyl-CoA reductase (NADPH). [EC: 1.3.1.34]

Predicted SEED Role

"2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)" (EC 1.3.1.34)

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.34

Use Curated BLAST to search for 1.3.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (666 amino acids)

>CSW01_09945 NADPH-dependent 2,4-dienoyl-CoA reductase (Vibrio cholerae E7946 ATCC 55056)
MYPNLFQPLDLGFTQLKNRVLMGSMHTGLEENKEGLHKLAAFYEERAKGGVGLIVTGGFS
PNLRGRLHPFSAEFSKTKHAKAHKVVTEAVHRHGAKIALQLLHAGRYAMHPFSQSASAIR
APIAKFAPSEMSTRQIRNTIQDFANSAELAQLAGYDGVEVMGSEGYLINQFICKRTNMRY
DEWGGSYQNRIRFPVEIVKAIREAVGKEFIIIFRLSMLDLVEQGSTFEEVVVLAKALEEA
GVTIINTGIGWHEARIPTIATQVPRAAFSWVTEKIKPYLKVPVVTCNRINTPEQAEKILA
SGQADMVSMARPFLADADFVRKAQEGQSALINTCIGCNQACLDNVFRGKRASCLVNPRAC
YETEIVVKPAQSKKIAVVGAGPAGLAFATTASERGHQVDLFERNDRIGGQFRLAMQIPGK
EEFRETIRYFANRIDQTGVKLHLGCEVQFSDLRGYDEVVIATGVTPRKIALAGLSESSKV
VDYQTLIREKTPVGQKVAIVGAGGIGVDVASMLTEPKDQTLDDWLYEWGIDKAIEHPGGL
YPYPETTSEREVWLLQRRKGAVGKGPGKTTGWIHKRTLEKRGVHLVGGVQYQKIDEQGLH
IERDGKSELIDADSVVICAGQESVRPFEAQWAELGDKLHVIGGADVAGELDAARAIRQGV
ELAVRL