Protein Info for CSW01_09720 in Vibrio cholerae E7946 ATCC 55056

Annotation: bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 31 to 146 (116 residues), 140.7 bits, E=2.5e-45
• PF02882: THF_DHG_CYH_C
  • amino acids 149 to 306 (158 residues), 227.8 bits, E=4.3e-72

Best Hits

Swiss-Prot: 100% identical to FOLD_VIBC3: Bifunctional protein FolD (folD) from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 100% identity to vch:VC1942)

MetaCyc: 71% identical to bifunctional methylenetetrahydrofolate dehydrogenase / methenyltetrahydrofolate cyclohydrolase (Escherichia coli K-12 substr. MG1655)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• folate transformations II (plants) (11/11 steps found)
• folate transformations III (E. coli) (9/9 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (3/3 steps found)
• folate transformations I (10/13 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (7/9 steps found)
• L-histidine degradation III (4/6 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (4/10 steps found)
• purine nucleobases degradation II (anaerobic) (12/24 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (24/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Protein Sequence (311 amino acids)

>CSW01_09720 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD (Vibrio cholerae E7946 ATCC 55056)
MPVDVIKNICDNVRKNFRSKPVRKVMTAQNIDGTLISQTVRSEVAARVKARVQAGLRAPG
LAVVLVGEDPASQVYVGSKRRACEEVGFVSKSFDLPATASEEALLSLVEELNNDPQIDGI
LVQLPLPAGMDTTKVLESIHPEKDVDGFHPYNVGRLAQRIPKLRSCTPKGIITLLERYNI
PLRGKHAVIVGASNIVGRPMTLELLLAGCTTTTCHRFTQDLEGHIRQADILVVAVGKPNF
IPGAWIKEGAVVVDVGINRLDTGKLVGDVEYDVARTRASFITPVPGGVGPMTVASLIENT
MMACEQFHTQG