Protein Info for CSW01_09395 in Vibrio cholerae E7946 ATCC 55056

Annotation: 3-deoxy-manno-octulosonate cytidylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 252 TIGR00466: 3-deoxy-D-manno-octulosonate cytidylyltransferase" amino acids 1 to 241 (241 residues), 385.2 bits, see alignment E=5.3e-120 PF02348: CTP_transf_3" amino acids 5 to 221 (217 residues), 242.3 bits, see alignment E=5.3e-76 PF12804: NTP_transf_3" amino acids 18 to 127 (110 residues), 47.7 bits, see alignment E=2e-16

Best Hits

Swiss-Prot: 100% identical to KDSB_VIBC3: 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)

KEGG orthology group: K00979, 3-deoxy-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase) [EC: 2.7.7.38] (inferred from 100% identity to vch:VC1875)

MetaCyc: 68% identical to 3-deoxy-manno-octulosonate cytidylyltransferase (Escherichia coli K-12 substr. MG1655)
3-deoxy-manno-octulosonate cytidylyltransferase. [EC: 2.7.7.38]

Predicted SEED Role

"3-deoxy-manno-octulosonate cytidylyltransferase (EC 2.7.7.38)" in subsystem KDO2-Lipid A biosynthesis (EC 2.7.7.38)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.38

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (252 amino acids)

>CSW01_09395 3-deoxy-manno-octulosonate cytidylyltransferase (Vibrio cholerae E7946 ATCC 55056)
MSFTVVIPARYQSTRLPGKPLADIGGKPMIQWVYEQAMQAGADRVIIATDDERVEQAVQA
FGGVVCMTSPNHQSGTERLAEVVAKMAIPADHIVVNVQGDEPLIPPAIIRQVADNLAACS
APMATLAVEIEDEAEVFNPNAVKVITDKSGYALYFSRATIPWDRDNFAKADKAIVQPLLR
HIGIYAYRAGFINTYLDWQPSQLEKIECLEQLRVLWHGEKIHVAVALEAPPAGVDTPEDL
EVVRRIVAERAQ