Protein Info for CSW01_09350 in Vibrio cholerae E7946 ATCC 55056

Annotation: formate C-acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01255: formate acetyltransferase
  • amino acids 8 to 758 (751 residues), 1343.1 bits, E=0
• PF02901: PFL-like
  • amino acids 20 to 612 (593 residues), 722.1 bits, E=7.4e-221
• PF01228: Gly_radical
  • amino acids 628 to 739 (112 residues), 125.4 bits, E=1.3e-40

Best Hits

Swiss-Prot: 83% identical to PFLB_ECOLI: Formate acetyltransferase 1 (pflB) from Escherichia coli (strain K12)

KEGG orthology group: K00656, formate C-acetyltransferase [EC: 2.3.1.54] (inferred from 88% identity to vsa:VSAL_I1955)

MetaCyc: 83% identical to pyruvate formate-lyase (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Pyruvate formate-lyase (EC 2.3.1.54)" in subsystem Butanol Biosynthesis or Fermentations: Mixed acid (EC 2.3.1.54)

MetaCyc Pathways

• superpathway of N-acetylneuraminate degradation (20/22 steps found)
• mixed acid fermentation (15/16 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (17/19 steps found)
• superpathway of L-threonine metabolism (15/18 steps found)
• L-threonine degradation I (6/6 steps found)
• pyruvate fermentation to acetate IV (3/3 steps found)
• pyruvate fermentation to ethanol I (3/3 steps found)
• reductive monocarboxylic acid cycle (2/2 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (7/9 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (6/13 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.54

Use Curated BLAST to search for 2.3.1.54

Protein Sequence (758 amino acids)

>CSW01_09350 formate C-acetyltransferase (Vibrio cholerae E7946 ATCC 55056)
MAEQFAKAWEGFAAGDWQNEVNVRDFIQKNYTPYEGDESFLVSEGTEATNKLWAKVMEGI
RQENSTHAPVDFDTSLISTITAHDAGYINKDLEKIVGLQTDAPLKRAIIPNGGIRMVEGS
CKAYGRELDPQVSKIYSEYRKTHNAGVFDIYTPEILACRKSGVLTGLPDAYGRGRIIGDY
RRVALYGIDFLMKDKLAQFKSLQEKFENGEDLQMTMQLREEIAEQHRALGQMKQMAAKYG
YDISRPAETAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSTFLDVYIERDMKAGKITEVEA
QEMIDHFVMKLRMVRFLRTPEYDELFSGDPIWATESMGGMGLDGRTLVTRTNFRFLNSLY
TMGPSPEPNITVLWSEQLPEGFKKFCAKVSIDTSSIQYENDDLMRPDFNNDDYAIACCVS
PMVIGKHMQFFGARANLAKTLLYVINGGVDEKLKIQVGPKMPKITDEVLDFDDVWGKLDH
FMGWLATQYVTALNAIHYMHDKYSYEAALMALHDRDVRRTMACGIAGLSVAADSLSAIKY
AKVKPIRDEDGVAIDFEIEGDYPKFGNNDSRVDDIACELVERFMNKIRSLKTYRNAVPTQ
SILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPMHGRDEKGAVASLTSVGKLPFAHAKD
GISYTFSIVPNALGKDENSQRANLAGLMDGYFHHEAGIEGGQHLNVNVLNRDTLLDAVKH
PEKYPQLTIRVSGYAVRFNSLTAEQQQDVIARTFTESL