Protein Info for CSW01_09240 in Vibrio cholerae E7946 ATCC 55056

Annotation: Holliday junction branch migration DNA helicase RuvB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 334 PF05496: RuvB_N" amino acids 25 to 183 (159 residues), 276.7 bits, see alignment E=1.6e-86 TIGR00635: Holliday junction DNA helicase RuvB" amino acids 28 to 331 (304 residues), 489.1 bits, see alignment E=2.2e-151 PF07728: AAA_5" amino acids 59 to 177 (119 residues), 30.7 bits, see alignment E=8.6e-11 PF00004: AAA" amino acids 60 to 184 (125 residues), 79.4 bits, see alignment E=1.1e-25 PF17864: AAA_lid_4" amino acids 186 to 259 (74 residues), 110.5 bits, see alignment E=7.8e-36 PF05491: RuvB_C" amino acids 261 to 330 (70 residues), 85.7 bits, see alignment E=5e-28

Best Hits

Swiss-Prot: 100% identical to RUVB_VIBCH: Holliday junction ATP-dependent DNA helicase RuvB (ruvB) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K03551, holliday junction DNA helicase RuvB (inferred from 100% identity to vco:VC0395_A1436)

MetaCyc: 80% identical to Holliday junction branch migration complex subunit RuvB (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Holliday junction DNA helicase RuvB" in subsystem DNA-replication or RuvABC plus a hypothetical

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (334 amino acids)

>CSW01_09240 Holliday junction branch migration DNA helicase RuvB (Vibrio cholerae E7946 ATCC 55056)
MIEADRLIAPISNHFKDEEVIDRAIRPKKLADYQGQDHVRDQMEIFIQAAQMRQEALDHL
LIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAALLTNLEENDVLFIDEIHRL
SPMVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGATTRAGSLTSPLRDRFGIV
QRLEYYKVADLQHIVQRSAQCLGLSMDSEGALEVARRARGTPRIANRLLRRVRDYAEVKG
DGHICAQTADRALNMLDVDHQGFDYMDRKLLLAIMEKFSGGPVGIDNLAAAIGEEKDTIE
DVLEPFLIQQGYLQRTPRGRIATDRAYLHFGIEK