Protein Info for CSW01_08635 in Vibrio cholerae E7946 ATCC 55056

Annotation: tRNA uridine 5-oxyacetic acid(34) methyltransferase CmoM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 260 PF13489: Methyltransf_23" amino acids 37 to 156 (120 residues), 32.9 bits, see alignment E=1.6e-11 PF01209: Ubie_methyltran" amino acids 38 to 151 (114 residues), 25.9 bits, see alignment E=1.9e-09 PF13847: Methyltransf_31" amino acids 49 to 154 (106 residues), 37.9 bits, see alignment E=4.4e-13 PF13649: Methyltransf_25" amino acids 51 to 146 (96 residues), 49.5 bits, see alignment E=1.7e-16 PF08241: Methyltransf_11" amino acids 52 to 148 (97 residues), 53.8 bits, see alignment E=7.5e-18 PF08242: Methyltransf_12" amino acids 52 to 148 (97 residues), 45.5 bits, see alignment E=3.3e-15

Best Hits

Swiss-Prot: 49% identical to CMOM_ECO57: tRNA 5-carboxymethoxyuridine methyltransferase (cmoM) from Escherichia coli O157:H7

KEGG orthology group: K06219, S-adenosylmethionine-dependent methyltransferase (inferred from 100% identity to vcm:VCM66_1657)

MetaCyc: 49% identical to tRNA cmo5U34 methyltransferase (Escherichia coli K-12 substr. MG1655)
2.1.1.M28 [EC: 2.1.1.M28]

Predicted SEED Role

"S-adenosylmethionine-dependent methyltransferase Functionally Coupled to the MukBEF Chromosome Partitioning Mechanism" in subsystem MukBEF Chromosome Condensation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.M28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (260 amino acids)

>CSW01_08635 tRNA uridine 5-oxyacetic acid(34) methyltransferase CmoM (Vibrio cholerae E7946 ATCC 55056)
MTEDRNFDDIAHKFAKNIYGSDKGEIRQVIVWEDLEQLLSQLDTQKVPLHVLDAGGGLAQ
VSQKIARLGHRVTLCDLSSEMLQLAEQDIANNGLLEQYRFVHSPVQTIQAHLDAPVDLVL
FHAVMEWLAEPKPALQNLLAQVRPGGMVSVMFYNYHGLVYKNAVCGNIPHVLEDMPHRKR
FKLQPQKGLLPEEVYQWIEESSFEICGKSGIRCFSDYIGNMKNMGEYQYEDLVALERKFC
RQEPYLSLGRYIHVWAKKKQ