Protein Info for CSW01_08595 in Vibrio cholerae E7946 ATCC 55056

Annotation: insulinase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 922 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00675: Peptidase_M16" amino acids 49 to 169 (121 residues), 97.8 bits, see alignment E=6e-32 PF05193: Peptidase_M16_C" amino acids 200 to 376 (177 residues), 91.8 bits, see alignment E=5e-30 amino acids 672 to 848 (177 residues), 71.9 bits, see alignment E=6.5e-24

Best Hits

KEGG orthology group: K07263, zinc protease [EC: 3.4.24.-] (inferred from 100% identity to vcm:VCM66_1649)

Predicted SEED Role

"Probable zinc protease pqqL (EC 3.4.99.-)" (EC 3.4.99.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-, 3.4.99.-

Use Curated BLAST to search for 3.4.24.- or 3.4.99.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (922 amino acids)

>CSW01_08595 insulinase family protein (Vibrio cholerae E7946 ATCC 55056)
MKIRLLVSILCILFAGCALQQTNRALQPDQRWVTQTLPNGLTYHLYPDSEQEVSIRLYVH
AGSMQETAQQAGYAHFIEHMAFNGTRHYQHNDVIRMFEQSGAQFGADFNALTGYDRTVYQ
LDLPNAQNIDKALLWFADIADGLAFDADEVEKEKGVILGEFRASRTENMSLEQQFYLHQI
QGTSYADRDPLGSRELVQAATPDSLKAFYQQWYQPQLAELVITGNFTLEQGQQWVENYFS
SWKKGSTEKPASIYHQALNNQDLVAPVTAGESPSLTLIFPQGSAAIKDYASQQEFWRDDV
GEQLLHTRLVAAFNDAAQAITGIYATHYEIEGQRYTLISVGFAAEQREKVQALLLETLAS
MRDYGVTKNELDIILRGYREHLTFLQEDREAITPASHANQKVYSIVFDTPIQATLDYQAS
LSEFIASATPEMINRHIQQQLSQNPVWVVGVAATEDAQALNKALPQWRNDLAQPGNQPID
QQIDSPFTQQFTAGEVVKQLDINDDPQVTYWQLDNGIDVYYLRNIEAKDRVFVQYASSGG
QFALPADLLPAAEIATAVQTRSGLDTLNGSQFDRYLRQKDIGFYSYIASTSHGFEANSKA
QELPELLEILHLLSTQVKVSPDQLNSVKTEFTQNRSAYFDSPIGAFFRTVTNQSFIETSP
YRIRTPEQIAQVTAQQIEQVHQRLFSEGRNNTLVIVGDIERSQITPMLRQYVASIPLSKG
TLSPMTSQLIKPVAPRLELALNNENSTQYSLRLISETQPRTAKTVFIDDMLQRIATQRLL
AEVREHQGLDYTPQVIPYVVDGDILNDWVLSALVDPKSEPQVAKVMHEVARELAQGVTQQ
ELDVVKQKFLIDMKPLNKSPEQQAYFMLRYAIHHYGVETIYKVEELTKSITLDDINQRAQ
TLFGKDTMSQELIMTPKANPKG