Protein Info for CSW01_08225 in Vibrio cholerae E7946 ATCC 55056

Annotation: ATP-dependent helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 594 PF04851: ResIII" amino acids 2 to 154 (153 residues), 98 bits, see alignment E=8.6e-32 PF00270: DEAD" amino acids 6 to 152 (147 residues), 47.4 bits, see alignment E=2.8e-16 PF00271: Helicase_C" amino acids 241 to 343 (103 residues), 60.8 bits, see alignment E=2.2e-20

Best Hits

Swiss-Prot: 60% identical to RADD_ECOLI: Putative DNA repair helicase RadD (radD) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to vcj:VCD_002739)

MetaCyc: 60% identical to putative DNA repair helicase RadD (Escherichia coli K-12 substr. MG1655)
Nucleoside-triphosphatase. [EC: 3.6.1.15, 3.6.1.5]

Predicted SEED Role

"ATP-dependent RNA helicase YejH"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (594 amino acids)

>CSW01_08225 ATP-dependent helicase (Vibrio cholerae E7946 ATCC 55056)
MYTLRPYQADSVKAVVHYFRQHSTPAVIVLPTGAGKSLVIAELARLARGRVLVLAHVKEL
VEQNHAKYEGYGLTGAIFSAGLGRKETDQQVVFASVQSVVRNLSEFQNQFSLLVIDECHR
VPDDKNSSYQKVITHLLELNPGMKVLGLTATPYRLGMGWIYQYHTRGLVRSEEPRFFRDC
IFELPIHYLLDEGFLTPAQLIDTPVMSYDFSQLKPANTGRYRESELDLVIEQSQRATPQI
VAQIIELAKARQGVMIFAATVRHAEEIYRLLPTEHAALVIGDTPTPERDRIIQAFKQQQI
KFLVNVSVLTTGFDAPHVDLIAILRPTESVSLYQQIVGRGLRLAAGKSDCLVLDYAGNQY
DLYQPEVGDPKPDSDSEIITIPCPACGFNNNFWGKLDANGFLIEHYGRRCQGYFTDDETG
EREHCGYRFRAKYCNECGADNDIAARICHECDTTLVDPDKKLKEALNLKDALVFECTDMS
LKVHKDAKGKSQLQVTYHGQDAVQVHQFWPLNTPRQKQQFHDQFVRPHLADKHRPFEAAT
PAKVVDHQHRFRPPLFVIARKSGRFWTIRDKVFADEFASPTDAHLSNRAYTIPT