Protein Info for CSW01_08215 in Vibrio cholerae E7946 ATCC 55056

Annotation: Bcr/CflA family drug resistance efflux transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 401 transmembrane" amino acids 16 to 37 (22 residues), see Phobius details amino acids 52 to 71 (20 residues), see Phobius details amino acids 83 to 102 (20 residues), see Phobius details amino acids 110 to 130 (21 residues), see Phobius details amino acids 142 to 166 (25 residues), see Phobius details amino acids 172 to 192 (21 residues), see Phobius details amino acids 221 to 247 (27 residues), see Phobius details amino acids 259 to 279 (21 residues), see Phobius details amino acids 289 to 309 (21 residues), see Phobius details amino acids 316 to 339 (24 residues), see Phobius details amino acids 351 to 371 (21 residues), see Phobius details amino acids 377 to 397 (21 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 17 to 395 (379 residues), 394.7 bits, see alignment E=3e-122 PF07690: MFS_1" amino acids 25 to 365 (341 residues), 192.3 bits, see alignment E=1.8e-60 PF06609: TRI12" amino acids 38 to 196 (159 residues), 43.5 bits, see alignment E=2.4e-15 PF00083: Sugar_tr" amino acids 56 to 195 (140 residues), 37.7 bits, see alignment E=1.8e-13

Best Hits

Swiss-Prot: 48% identical to BCR_ECOLI: Bicyclomycin resistance protein (bcr) from Escherichia coli (strain K12)

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 100% identity to vcm:VCM66_1573)

MetaCyc: 48% identical to multidrug efflux pump Bcr (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-340; TRANS-RXN-44

Predicted SEED Role

"MFS family multidrug transport protein, bicyclomycin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (401 amino acids)

>CSW01_08215 Bcr/CflA family drug resistance efflux transporter (Vibrio cholerae E7946 ATCC 55056)
MSASTVNTQQTPQMSLLLYIVLGALGALTPLAIDMYLPAMPTIARDFGVNAGAVQITLTA
YTAGFAIGQLLHGPLADSFGRRPVMLCGIFLFGIAAMVSATTNGIEALTYIRAAQGFAGA
AAAVVIQAVVRDMFDREDFARAMSFVTLVVTLAPLVAPMIGGHLAIWFGWRSIFWVLALF
AALVIAMVMWKIPETLSPENRQPLRFRSTMRNYFDLCKNPVAMGLIFSGAFSFAGMFAFL
TAGAFVYIDIYGVSPDGFGYLFGLNIVAMIALTTINGRFVKKIGSHNMLRFALFLQLMAG
IGLFVSWVLDWGLWGTVPFVVLFVGTISTIGSNSMGLLLSKYPRMAGTASSLAGTLRFGT
GSIIGAVVAALPGTETWPMIFVMAACSVLSGAFYWTLGRKA