Protein Info for CSW01_08115 in Vibrio cholerae E7946 ATCC 55056

Annotation: ribosomal RNA large subunit methyltransferase F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 362 PF05971: Methyltransf_10" amino acids 44 to 350 (307 residues), 516.8 bits, see alignment E=9.8e-160

Best Hits

Swiss-Prot: 100% identical to RLMF_VIBCH: Ribosomal RNA large subunit methyltransferase F (rlmF) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K06970, ribosomal RNA large subunit methyltransferase F [EC: 2.1.1.181] (inferred from 100% identity to vcm:VCM66_1554)

Predicted SEED Role

"Ribosomal RNA large subunit methyltransferase F (EC 2.1.1.51)" (EC 2.1.1.51)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.51

Use Curated BLAST to search for 2.1.1.181 or 2.1.1.51

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (362 amino acids)

>CSW01_08115 ribosomal RNA large subunit methyltransferase F (Vibrio cholerae E7946 ATCC 55056)
MNTPLKPKHGQKTNRKPKANKPVVKKQQTKQPPTHKVQGEEVAAVKSGLHPRNRHRGQYD
FPALIKVVPELQSHVMKNPKGQWTINFADPISVKLLNKALLVLHYGVTYWDIPEGFLCPP
IPGRADYIHRVADLLLKGNPQLNHSQVRALDIGVGANCIYPIVGVTEYGWSWVGSDVDPV
SIQQASLIVQSNSKLQGHIECRLQKNSQHIFNGIIGANERYTVTTCNPPFHASLADAQQG
TQRKLTNLQANQRKKGRLATPTLSHSRLNFGGQKAELWCPGGEAAFIGKMAVESQQFAQQ
VLWFRTLISKGDNVRGMKKQLEKLGAQSIHVIEMAQGQKISRFIAWSFQNAEQRKLWWQA
KC