Protein Info for CSW01_07545 in Vibrio cholerae E7946 ATCC 55056

Annotation: 16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 503 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details PF17125: Methyltr_RsmF_N" amino acids 37 to 130 (94 residues), 95.6 bits, see alignment E=4.8e-31 TIGR00446: NOL1/NOP2/sun family putative RNA methylase" amino acids 70 to 338 (269 residues), 329.9 bits, see alignment E=5e-103 PF01189: Methyltr_RsmB-F" amino acids 146 to 336 (191 residues), 165.1 bits, see alignment E=4.3e-52 PF21150: YebU_pre-PUA_dom" amino acids 356 to 433 (78 residues), 100.3 bits, see alignment E=1.1e-32 PF13636: Methyltranf_PUA" amino acids 449 to 498 (50 residues), 42.8 bits, see alignment 1.1e-14

Best Hits

Swiss-Prot: 100% identical to RSMF_VIBCM: Ribosomal RNA small subunit methyltransferase F (rsmF) from Vibrio cholerae serotype O1 (strain M66-2)

KEGG orthology group: K11392, ribosomal RNA small subunit methyltransferase F [EC: 2.1.1.-] (inferred from 100% identity to vco:VC0395_A1110)

MetaCyc: 57% identical to 16S rRNA m5C1407 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11593 [EC: 2.1.1.178]

Predicted SEED Role

"Ribosomal RNA small subunit methyltransferase F (EC 2.1.1.-)" (EC 2.1.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.178

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (503 amino acids)

>CSW01_07545 16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF (Vibrio cholerae E7946 ATCC 55056)
MCCFSTFRRSFMSPPYLVIIAALIHLRNSLVHPNISLPEPFIASMAKILPDPTQLADFIA
ACQRPLRKSIRVNTLKISVAEFCQRAAEKEWQLTPVPWCENGFWIDADESLVPLGNTAEH
MAGLFYIQEASSMMPVSALFMGNAHYDSVLDMAAAPGSKTTQMAALMDNQGVLVANEFSA
SRVKVLHANIERCGIRNTALSNFDGCVFGGWLPERFDAVLIDAPCSGEGTIRKDPDAMKN
WSLESIQSIANTQKALIESAFQALKVGGTLVYSTCTLSREENQQVCWHLKQTYGDAVSFE
SLGNLFEHASLALTEEGFLHIFPQMYDCEGFFVAKIRKLASVPTPEVNKRLGKFPFNKAS
HKQQAEIAQQLHKSLGIELPSDAQVWLRDNDVWLFPEALEPLLGELRFSRMGIKIAEAHK
SGYRWQHQVATCLASSSASHSVELDTTQAREWFMGRDVRPEGQSGQGEVIIRYANDVIGL
GKWVGNRVKNGLPRELVRDKNLF