Protein Info for CSW01_07495 in Vibrio cholerae E7946 ATCC 55056

Annotation: NAD-glutamate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21075: GDH_ACT1
  • amino acids 36 to 172 (137 residues), 157 bits, E=1.2e-49
• PF21073: GDH_HM1
  • amino acids 340 to 399 (60 residues), 85.4 bits, see alignment (E = 7.7e-28)
• PF21076: GDH_ACT2
  • amino acids 403 to 492 (90 residues), 117 bits, 1.8e-37
• PF21079: GDH_HM2
  • amino acids 498 to 542 (45 residues), 62.8 bits, see alignment (E = 9.5e-21)
• PF21077: GDH_ACT3
  • amino acids 548 to 627 (80 residues), 97.4 bits, 1.3e-31
• PF21078: GDH_HM3
  • amino acids 644 to 709 (66 residues), 105.2 bits, see alignment (E = 6.2e-34)
• PF05088: Bac_GDH_CD
  • amino acids 725 to 1219 (495 residues), 896.4 bits, E=1.8e-273
• PF21074: GDH_C
  • amino acids 1264 to 1603 (340 residues), 409.8 bits, E=3.8e-126

Best Hits

KEGG orthology group: K00260, glutamate dehydrogenase [EC: 1.4.1.2] (inferred from 58% identity to avr:B565_1958)

Predicted SEED Role

"NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form" in subsystem Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.2)

MetaCyc Pathways

• L-alanine degradation II (to D-lactate) (3/3 steps found)
• L-glutamate degradation I (1/1 steps found)
• ethene biosynthesis IV (engineered) (1/3 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• 4-aminobutanoate degradation V (2/7 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (2/7 steps found)
• methylaspartate cycle (9/19 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.2

Protein Sequence (1613 amino acids)

>CSW01_07495 NAD-glutamate dehydrogenase (Vibrio cholerae E7946 ATCC 55056)
MTARETLMPVLLEKVYQLIQDKLELAQQPLVTQLGQHLFSNISQDDLVERNESDLYGAVL
SLWHHINEKKADERSVRVFNPTVSRQGWQSTHTIVEIVLPDSPFLVDSIKMALSRLGLAS
HLMLNGPAHIARHDDGSIKSINQGEGQLTSMFHIEVDRLSSKEEMTELKNELLDILHDTA
LVVKDWKPMATKLEQVINQLEADKKQIPVEAERLQETIQFLRWLGNHNFTFMGYKEFDLV
EKNGDTELTPTKDTGLGLFSDNERVRSVKLSQFPDSARLEAKKPFLLILTKGNKQSRIHR
PAYTDYIGIKKFDAKGKVIGEHRFTGLYTSAVYNQSVEGIPLIREKVGRILAASGYRQGS
YAYKALHNILENYPRDELLQAREEELLEVGMGVVQMQDRDLLRLFVRKDPFGRFFSCMVY
VTKERYNTELRRKTQQVFKQYFGCEQDVEFTTYFSESPLARTHYIVRVDNNNINVDVKKI
EQNLMEASTSWDDRLAEAIVANFGESRGLPLSKEYQRAFPRSYKEDVMPGSALADIEHLE
ALDEHNKLGMLFYRLQETAKDSKAVRLKLYHKDEPIHLSDVMPMLENLGLRVIGESPYEV
VKANGQVYWILDFSMLHKSDKQVDLREARDRFQQAFAAIWAGELESDGFNRLILGASLSG
REVSILRAYARYMRQVGFPFSQHYIEDTLSHHPDLAQGLVDLFVRRFDPKYKGGEKGQAE
IIKSLTEQLDQVQSLDDDRIIRRYMEMINATLRTNYYQLDEHKQNKPWLSLKMKPSEIPE
IPAPVPAFEIFVYAPDIEGVHLRGGKVARGGLRWSDRQEDFRTEILGLVKAQQVKNTVIV
PVGAKGGFVCKKQYLYTTRDEIFAEGQRCYKRFIRALLDVTDNIIEGQVVPPKNVVRHDE
DDPYLVVAADKGTATFSDLANSVSAEYQFWLGDAFASGGSNGYDHKAMGITAKGGWESVK
RHFREMGIDCQTTDFTAIGIGDMAGDVFGNGMLLSKHIRLLAAFNHIHIFIDPTPDSASS
WEERNRLFNLPRSSWEDYNPKLISKGGGVFSRKAKAITLTPEMQKMLNTKKTTLAPNELI
KMILKMEVDLLWNGGIGTYVKSSIETHTDVGDRANDGLRVDGREVNAKIIGEGGNLGMTQ
RGRIEFALKGGRVNTDFVDNVGGVDCSDNEVNIKIFLNGLVANGDLTLKQRNQILESMKD
EVGSIVIEDAYGQSESISVTEAQGVSLMKEQIRFIHHMEKAGYLDRALEHIPDDETLLER
ERQGMGLTRPELSVLMAYGKMALKEELASEEIAQDEFHAKQLVNYFPTELRGHYAQQMVN
HPLRVEIIATALANQMVNEMGCNFVTRLQEETGSSVVDIANAYAAAREIYGLGIVLEKVR
KLDNIAQSSAQYDVMFLVRRTLRRLTRWLLRNRTGKPSVIAMVERYQEDVKAITEQLDKV
LVKEEIVEHNSMAENWIEKGIEKELAHYVARLSSLYSVLDISAVAKEKGIAVTQTAKLYF
HLGDRLSLHWFLKQINHQAVDNHWQALARASFREDLDWQQRQLTAQVLSSNLSDAQQEIE
LALDKWLERNQVSISRWENILSEFKVGTVHEFAKFSVALRELTLLNLNCLTVE