Protein Info for CSW01_07490 in Vibrio cholerae E7946 ATCC 55056

Annotation: dihydroorotate dehydrogenase (quinone)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to PYRD_VIBCH: Dihydroorotate dehydrogenase (quinone) (pyrD) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 100% identity to vco:VC0395_A1098)

MetaCyc: 68% identical to dihydroorotate dehydrogenase, type 2 (Escherichia coli K-12 substr. MG1655)
Dihydroorotate dehydrogenase. [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]; 1.3.5.2 [EC: 1.3.5.2]

Predicted SEED Role

"Dihydroorotate dehydrogenase (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
UMP biosynthesis I (6/6 steps found)
UMP biosynthesis II (6/6 steps found)

KEGG Metabolic Maps

Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.3.1 or 1.3.5.2 or 1.3.98.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (336 amino acids)

>CSW01_07490 dihydroorotate dehydrogenase (quinone) (Vibrio cholerae E7946 ATCC 55056)
MLYRLARAGFFQLDAEKAHDLAISNFKRFTGTPFDLFYRQQLPHRPVQCMGLTFKNPVGL
AAGLDKNGECIEAFGAMGFGFVEVGTVTPRPQAGNDKPRLFRLVHAEGIINRMGFNNLGV
DHLVENVKRAKYDGIIGINIGKNKDTPIEKGAEDYLICMDKVYPYAGYIAVNISSPNTPG
LRSLQYGEALDELLAALKTRQAELAAKHDKYVPLALKIAPDLSDDEIQQICQSLLKNKID
SVIATNTTLDRSLVEGMKFANEAGGLSGRPLQNRSTEVIKCLYKELGEEIPIIGVGGIDS
YISAKEKLLAGAKLVQVYSGFIYQGPGLVADIVKNL