Protein Info for CSW01_07450 in Vibrio cholerae E7946 ATCC 55056

Annotation: Lon protease family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 598 transmembrane" amino acids 28 to 46 (19 residues), see Phobius details PF13654: AAA_32" amino acids 129 to 238 (110 residues), 31.4 bits, see alignment E=1.8e-11 PF05362: Lon_C" amino acids 449 to 553 (105 residues), 40.5 bits, see alignment E=2.4e-14

Best Hits

KEGG orthology group: K04770, Lon-like ATP-dependent protease [EC: 3.4.21.-] (inferred from 100% identity to vcm:VCM66_1425)

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type II" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-, 3.4.21.53

Use Curated BLAST to search for 3.4.21.- or 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (598 amino acids)

>CSW01_07450 Lon protease family protein (Vibrio cholerae E7946 ATCC 55056)
MLYSDSVTHCLQTCNSPYQKSEKDHRRLSLFAARLFLYNLALFNQFDISMTQSIWRSVTP
QFEQFNKVFENYPNLAPISLIEIQPRLTSALEQFVHLKGFSRILLINAPDNSIYRSLFKD
ALLEQDLDVPVVSTETLDISEILGQVKVEHGGLVHKTSGYLEQANGGYLIVSANLLLANP
RYWPTVKAAVLGETVSPINCDPKSPLPNRLGSSYDVKLIVVGDRTQLGDLDFLDADIHSG
LCLFSELELDLKIEEETIEDYLGYLRWLCEQYNLPSLTCSAIQSLMTAGARYTEDQYYAP
LCLMWHRALLEEARLESEEPLIDAEHIEIALDKRYLRESYLPERALDDILDGQVIIETQG
EQVGQVNGLTVIEVTGHPMPYGEPARISCVIHFGDGDIADVERKVELGGNLHAKGMMIMQ
AFVSSALNLDGSLPFSASIVFEQSYSEVDGDSASLAELCSLVSALSEYPIDQQIAVTGAV
DQFGRVQAVGGLNEKIEGFYRVCCHQGLTGEQGVILPSSNLKHLALHKSVVESIKNGEFN
IWPVSTVDEAIPLLMGKPFRGEDEDSVIAKIAERIDNFEKLVQPHGIVERIKNWLSWH