Protein Info for CSW01_07145 in Vibrio cholerae E7946 ATCC 55056

Annotation: spermidine/putrescine ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 343 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF02030: Lipoprotein_8" amino acids 11 to 109 (99 residues), 43.5 bits, see alignment E=5e-15 PF01547: SBP_bac_1" amino acids 37 to 282 (246 residues), 91.9 bits, see alignment E=2e-29 PF13531: SBP_bac_11" amino acids 42 to 292 (251 residues), 39.2 bits, see alignment E=1.7e-13 PF13416: SBP_bac_8" amino acids 43 to 301 (259 residues), 128.7 bits, see alignment E=9.7e-41 PF13343: SBP_bac_6" amino acids 77 to 302 (226 residues), 81.8 bits, see alignment E=1.4e-26

Best Hits

Swiss-Prot: 58% identical to POTD_SALTY: Spermidine/putrescine-binding periplasmic protein (potD) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K11069, spermidine/putrescine transport system substrate-binding protein (inferred from 100% identity to vco:VC0395_A1036)

MetaCyc: 58% identical to spermidine preferential ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]; 7.6.2.11 [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"ABC transporter, periplasmic spermidine putrescine-binding protein PotD (TC 3.A.1.11.1)" in subsystem Polyamine Metabolism (TC 3.A.1.11.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (343 amino acids)

>CSW01_07145 spermidine/putrescine ABC transporter substrate-binding protein (Vibrio cholerae E7946 ATCC 55056)
MKKLATLLAGSACALSLLSGTAAAEDKELVFMNWGPYINSGILEQFTKETGIKVIYSTYE
SNETLYAKLKTHNQGYDLVVPSTYFVAKMRDEGMLQKIDKSKLSNFKNLDSNYLDKPYDP
NNDYSIPHVVAITGLAVNTDMYDPNDFQSWGDLWKPELKGQLMLMDDTREVFHIALRKLG
YSGNSTDDKQIDEAYAELQKLMPNVLVFNSDNPAAPYLSGEVGLGMLWNGSAAAAQKEGL
PIKLVFPKEGGIGWVDNFAISSGAKNVDAAHKMIDFLLRPEIAEQISRDTGYLTGVKASN
DKFQDVAPLFPSQEDLDRVEWQSAVGDKTVKYEEYFMKLKAGQ